Recombinant Human R-Spondin 3 Protein, CF


Recombinant Human R-Spondin 3 (Catalog # 3500-RS/CF), in the presence of Recombinant Mouse Wnt 3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay. The more

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human R-Spondin 3 Protein, CF Summary

Details of Functionality
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.5‑2.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 3 protein
Accession #
N-terminal Sequence
No results obtained: Gln22 predicted
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
28.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
36-45 kDa, reducing conditions
Read Publications using
3500-RS/CF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human R-Spondin 3 Protein, CF

  • Cristin 1
  • FLJ14440
  • hPWTSR
  • hRspo3
  • Protein with TSP type-1 repeat
  • Roof plate-specific spondin-3
  • RSPO3
  • R-spondin 3 homolog (Xenopus laevis)
  • RSpondin 3
  • R-Spondin 3
  • R-spondin-3
  • Thrombospondin type-1 domain-containing protein 2
  • thrombospondin, type I, domain containing 2
  • THSD2


R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31 kDa secreted protein that shares ~40% amino acid (aa) identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt/ beta -catenin signaling, but each has a distinct expression pattern (1-4). Like other R-spondins,R-Spondin 3 contains two adjacent cysteine-rich furin-like domains (aa 35-135) with one potential N-glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-269). Only the furin-like domains are needed for beta -catenin stabilization (2). Within aa 21-209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92% aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279 and 297 aa diverge at aa 210 and 276, respectively (5). Mouse R-Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta-specific transcription factor, Gcm1. In the mouse embryo, R-Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R-Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (8-10). R-Spondin 3 has also been identified as an oncogene (11).

  1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.
  2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.
  3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.
  4. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
  5. Entrez Accession # EAW48114 and EAW48116.
  6. Kazanskaya, O. et al. (2008) Development 135:3655.
  7. Aoki, M. et al. (2007) Dev. Biol. 301:218.
  8. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
  9. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
  10. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  11. Theodorou, V. et al. (2007) Nat. Genet. 6:759.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
Species: Mu
Applications: BA
Species: Hu
Applications: BA
Species: Ch, Av-Du, Hu, Mu, Pm, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu
Applications: WB
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IP, WB
Species: Hu
Applications: WB
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu, Rt
Applications: IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu, Mu, Pm, Rt
Applications: WB
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: ICC, WB
Species: Hu
Applications: BA

Publications for R-Spondin 3 (3500-RS/CF)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Chlorocebus pygerythrus (Vervet Monkey).

We have publications tested in 3 applications: Bioassay, Cell Culture, IHC Control.

Filter By Application
Cell Culture
IHC Control
All Applications
Filter By Species
Primate - Chlorocebus pygerythrus (Vervet Monkey)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using 3500-RS/CF Applications Species
G Pizzolato, L Moparthi, S Söderholm, C Cantù, S Koch The oncogenic transcription factor FOXQ1 is a differential regulator of Wnt target genes Journal of Cell Science, 2022;0(0):. 2022 [PMID: 36124643] (Cell Culture, Human) Cell Culture Human
JF Dekkers, EJ van Vliet, N Sachs, JM Rosenbluth, O Kopper, HG Rebel, EJ Wehrens, C Piani, JE Visvader, CS Verissimo, SF Boj, JS Brugge, H Clevers, AC Rios Long-term culture, genetic manipulation and xenotransplantation of human normal and breast cancer organoids Nature Protocols, 2021;0(0):. 2021 [PMID: 33692550] (Bioassay, Human) Bioassay Human
C Beaurivage, A Kanapeckai, C Loomans, KS Erdmann, J Stallen, RAJ Janssen Development of a human primary gut-on-a-chip to model inflammatory processes Scientific Reports, 2020;10(1):21475. 2020 [PMID: 33293676] (Bioassay, Human) Bioassay Human
X Li, B Pan, X Song, N Li, D Zhao, M Li, Z Zhao Breast cancer organoids from a patient with giant papillary carcinoma as a high-fidelity model Cancer Cell Int., 2020;20(0):86. 2020 [PMID: 32206037] (Bioassay, Human) Bioassay Human
M Zhang, M Haughey, NY Wang, K Blease, AM Kapoun, S Couto, I Belka, T Hoey, M Groza, J Hartke, B Bennett, J Cain, A Gurney, B Benish, P Castiglion, C Drew, J Lachowicz, L Carayannop, SD Nathan, J Distler, DA Brenner, K Hariharan, H Cho, W Xie Targeting the Wnt signaling pathway through R-spondin 3 identifies an anti-fibrosis treatment strategy for multiple organs PLoS ONE, 2020;15(3):e0229445. 2020 [PMID: 32160239] (IHC Control, Human) IHC Control Human
PG Boone, LK Rochelle, JD Ginzel, V Lubkov, WL Roberts, PJ Nicholls, C Bock, ML Flowers, RJ von Furste, BR Stripp, P Agarwal, AD Borowsky, RD Cardiff, LS Barak, MG Caron, HK Lyerly, JC Snyder A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion Nat Commun, 2019;10(1):5490. 2019 [PMID: 31792216] (Cell Culture, Human) Cell Culture Human
S Park, J Cui, WA Yu, L Wu, K Carmon, QJ Liu Differential activities and mechanisms of the four R-Spondins in potentiating Wnt/?-catenin signaling J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29752411] (Bioassay, Human) Bioassay Human
AM Lebensohn, R Rohatgi R-spondins can potentiate WNT signaling without LGRs Elife, 2018;7(0):. 2018 [PMID: 29405118] (Bioassay, Human) Bioassay Human
D Zeve, DP Millay, J Seo, JM Graff Exercise-Induced Skeletal Muscle Adaptations Alter the Activity of Adipose Progenitor Cells PLoS ONE, 2016;11(3):e0152129. 2016 [PMID: 27015423] (Bioassay, Primate - Chlorocebus pygerythrus (Vervet Monkey)) Bioassay Primate - Chlorocebus pygerythrus (Vervet Monkey)
Chartier C, Raval J, Axelrod F, Bond C, Cain J, Dee-Hoskins C, Ma S, Fischer M, Shah J, Wei J, Ji M, Lam A, Stroud M, Yen W, Yeung P, Cancilla B, O'Young G, Wang M, Kapoun A, Lewicki J, Hoey T, Gurney A Therapeutic Targeting of Tumor-Derived R-Spondin Attenuates beta-Catenin Signaling and Tumorigenesis in Multiple Cancer Types. Cancer Res, 2016;76(3):713-23. 2016 [PMID: 26719531] (Bioassay, Mouse) Bioassay Mouse
Show All 14 Publications.

Reviews for R-Spondin 3 (3500-RS/CF) (0)

There are no reviews for R-Spondin 3 (3500-RS/CF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for R-Spondin 3 (3500-RS/CF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional R-Spondin 3 Products

Bioinformatics Tool for R-Spondin 3 (3500-RS/CF)

Discover related pathways, diseases and genes to R-Spondin 3 (3500-RS/CF). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for R-Spondin 3 (3500-RS/CF)

Discover more about diseases related to R-Spondin 3 (3500-RS/CF).

Pathways for R-Spondin 3 (3500-RS/CF)

View related products by pathway.

PTMs for R-Spondin 3 (3500-RS/CF)

Learn more about PTMs related to R-Spondin 3 (3500-RS/CF).

Blogs on R-Spondin 3

There are no specific blogs for R-Spondin 3, but you can read our latest blog posts.
Omicron Variant Antibodies

Contact Information

Download our Western Blot eHandbook

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human R-Spondin 3 Protein, CF and receive a gift card or discount.


Gene Symbol RSPO3