Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PGLYRP4/PGRP-I beta Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When peptidoglycan is coated at 1 μg/mL (100 μL/well), the concentration of Recombinant Human PGLYRP4/PGRP-I beta that produces 50% optimal binding response is 0.5-3 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human PGLYRP4/PGRP-I beta protein
  Asp18-His373 with a C-terminal 6-His tag
• Accession #: AAK72485
• N-terminal Sequence: Asp18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Enzymes
• Gene: PGLYRP4
• Purity: >95%, by SDS-PAGE with silver staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 53-64 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE with silver staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PGLYRP4/PGRP-I beta Protein, CF

• peptidoglycan recognition protein 4
• peptidoglycan recognition protein I beta
• Pglyrp-1 beta
• PGLYRP4
• PGRPI beta
• PGRP-I beta
• PGRP-I-beta

Background

PGLYRP4, also known as PGRP-I beta , is a member of the peptidoglycan recognition protein family of innate immunity proteins (1, 2). Human PGLYRP4 is expressed in the skin, eyes, salivary glands, throat, tongue, esophagus, stomach, and intestine (3). Mature human PGLYRP4 contains two nonidentical PGRP domains, and it shares 77% and 74% amino acid sequence identity with mouse and rat PGLYRP4, respectively (1, 4). It is secreted as disulfide-linked homodimers and binds peptidoglycan (PGN) and PGN-containing Gram-positive bacteria (1, 3). PGLYRP4 is directly bactericidal against pathogenic and nonpathogenic Gram-positive bacteria, but not normal flora bacteria, suggesting that normal flora bacteria have developed resistance to this bactericidal mechanism (3, 5, 6). Its bactericidal activity requires physiological concentrations of Zn²⁺ (6). PGLYRP4 knockout mice are more sensitive to the development of experimental dermatitis and DSS-induced colitis than wild type mice (2, 7). In humans, PGLYRP4 single nucleotide polymorphisms have been associated with inflammatory bowel disease and increased Parkinson’s disease risk (8, 9).

1. Liu, C. et al. (2001) J. Biol. Chem. 276:34686.
2. Park, S.Y. et al. (2011) PLoS One 6:e24961.
3. Lu, X. et al. (2006) J. Biol. Chem. 281:5895.
4. Royet, J. and R. Dziarski (2007) Nat. Rev. Microbiol. 5:264.
5. Tydell, C.C. et al. (2006) J. Immunol. 176:1154.
6. Wang, M. et al. (2007) J. Immunol. 178:3116.
7. Saha, S. et al. (2010) Cell Host Microbe 8:147.
8. Zulfiqar, F. et al. (2013) PLoS One 8:e67393.
9. Goldman, S.M. et al. (2014) Mov. Disord. 29:1171.

Bioinformatics

• Gene Symbol: PGLYRP4
• Uniprot:
  • AAK72485()