Recombinant Human Osteocrin Protein, CF

When Recombinant Human NPRC/NPR-3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 μg/mL, Recombinant Human Osteocrin (Catalog # 9669-ON) binds with an ED50 of 2‑20 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Osteocrin Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human NPRC/NPR3 Fc Chimera (Catalog # 10233-NR) is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Osteocrin that produces 50% of the optimal binding response is 2-20 ng/mL.
  
• Source: E. coli-derived human Osteocrin protein
  Val28-Gly133, with a C-terminal 6-His tag
• Accession #: P61366
• N-terminal Sequence: Val28
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 13 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 18 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Osteocrin Protein, CF

• Musclin
• Osteocrin
• OSTN

Background

Osteocrin, also known as Musclin, is a secreted protein that is primarily expressed in bone and muscle. It is synthesized as a proprotein (11 kDa) that undergoes proteolytic processing to generate a mature 50 amino acids (5 kDa) C-terminal peptide (1). It was found to modulate osteoblast differentiation and to regulate glucose metabolism in muscles (2). Human Osteocrin proprotein shares 77% and 78% amino acid sequence identity with the rat and mouse protein, respectively. Secretion of Osteocrin has been shown to be increased with exercise and is associated with metabolically beneficial formation of brown fat (3). Osterocrin has also been attributed to increases in exercise endurance through promotion of mitochondrial biosynthesis (4). Based on similarities with NPs (natriuretic peptides), Osteocrin was found to interact with NP clearance receptor NPR-C. This interaction is thought to modulate the availability of NPs, importantly increasing CNP which has been shown to stimulate endochondral ossification and elongate bones (5). In primates, Osteocrin may have evolved to regulate neuronal structure and function (6).

1. Nishizawa, H. et al. (2004) J Bio Chem 279:19391.
2. Thomas, G. et al. (2003) J Bio Chem 278:50563.
3. Jeramic, N. et al. (2017) J Cell Physiol 232:61.
4. Subbatino, E. et al. (2015) Proc Natl Acad Sci U S A 112:16042.
5. Kanai, Y. et al. (2017) J of Clin Invest 127:4136.
6. Ataman, B. et al. (2016) Nature 539:242.

Publications for Osteocrin (9669-ON)(1)

Publication using 9669-ON

• X Zhang, C Hu, XP Yuan, YP Yuan, P Song, CY Kong, T Teng, M Hu, SC Xu, ZG Ma, QZ Tang Osteocrin, a novel myokine, prevents diabetic cardiomyopathy via restoring proteasomal activity Cell Death & Disease, 2021-06-16;12(7):624. 2021-06-16 [PMID: 34135313] (In Vivo, Mouse)

Bioinformatics

• Uniprot:
  • P61366()