Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Nogo-A protein
  

  Human Nogo-A (Val566 - Phe748) & (Thr569 - Phe748) Accession # Q9NQC3	IEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: Q9NQC3
• N-terminal Sequence: Val566 & Thr569
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: RTN4
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Nogo-A (aa 566-748) Fc Chimera Protein, CF

• ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X
• NI220
• NogoA
• Nogo-A
• RTN4
• RTN4A

Background

Human Nogo-A (also reticulon-4) is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence-containing N-terminus, a topologically conserved 200 amino acid (aa) C-terminus that contains two transmembrane domains with an ER-retention motif, and a punctate intracellular distribution within the ER that is reminescent of a reticulum (1 - 4). In human, Nogo exists in five isoforms (5 - 7). The full length human form (Nogo-A) is 1192 aa in length and contains a 1018 aa N-terminus, a 21 aa transmembrane segment, a 94 aa connecting “loop”, a second 21 aa transmembrane segment, and a 38 aa C-terminus. Three areas are of particular interest. One is a stretch of 66 aa within the 94 aa connecting loop. This segment is reported to bind to the GPI-linked Nogo receptor/p75 complex on axons and induce growth cone collapse (8 - 10). Two other areas in the N-terminus have also been discovered to have bioactivity (8, 11, 12). Based on rat, aa 57 - 184 in human (aa 59 - 172 in rat) should block fibroblast spreading, while aa 566 - 748 in human (aa 544 - 725 in rat) block neurite outgrowth and block fibroblast spreading (8, 12, 13). The exact topology of Nogo-A is unclear. The N- and C-termini may be extracellular with the “loop” region intracellular, or the situation could be reversed (13 - 15). Alternatively, the loop region and N-terminus may be on the same side of the membrane (3, 8). The four additional isoforms are shorter than Nogo-A (199 aa [Nogo-C], 373 aa [Nogo-B], 392 aa and 986 aa, respectively) (7). Although highly divergent, all contain the same C-terminal stretch, aa 1005 - 1192. Both Nogo-B and C are reported to complex with Nogo-A (16). Notably, Nogo-A is expressed in neurons, endothelial cells. oligodendrocytes, fibroblasts and myoblasts (12, 16 - 18). Human Nogo-A is 78% aa identical to mouse and rat Nogo-A overall, with 98% aa identity in the loop region and approximately 80% aa identity in the aa 566 - 748 segment.

1. Oertle, T. et al. (2003) FASEB J. 17:1238.
2. GrandPre, T. et al. (2000) Nature 403:439.
3. Yan, R. et al. (2006) Cell. Mol. Life Sci. 63:877.
4. Teng, F.Y.H. et al. (2004) J. Neurochem. 89:801.
5. Chen, M.S. et al. (2000) Nature 403:434.
6. Morris, N.J. et al. (1999) Biochim. Biophys. Acta 1450:68.
7. GenBank Accession # Q9NQC3.
8. Oertle, T. et al. (2003) J. Neurosci. 23:5393.
9. Fournier, A.E. et al. (2001) Nature 409:341.
10. Wang, K.C. et al. (2002) Nature 420:74.
11. Prinjha, R. et al. (2000) Nature 403:384.
12. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
13. Li, M. et al. (2004) Eur. J. Biochem. 271:3512.
14. Huber, A.B. and M.E. Schwab (2000) Biol. Chem. 381:407.
15. Ng, C.E.L. and B.L. Tang (2002) J. Neurosci. Res. 67:559.
16. Dodd, D.A. et al. (2005) J. Biol. Chem. 280:12494.
17. Wang, X. et al. (2002) J. Neurosci. 22:5505.
18. Acevedo, L. et al. (2004) Nat. Med. 10:382.

Bioinformatics

• Gene Symbol: RTN4
• Uniprot:
  • Q9NQC3()