Recombinant Human MEGF9 His-tag Protein, CF

2 μg/lane of Recombinant Human MEGF9 His-tag (Catalog # 10514-ME) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 136-150 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MEGF9 His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human MEGF9 protein
  Ala36-Asn514, with a C-terminal 6-His tag
• Accession #: Q9H1U4.3
• N-terminal Sequence: Ala36
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 136-150 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 µg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MEGF9 His-tag Protein, CF

• EGFL5
• EGFL5KIAA0818
• EGF-like protein 5
• EGF-like-domain, multiple 5
• Epidermal growth factor-like protein 5
• MEGF9
• Multiple EGF-like domains protein 9
• multiple EGF-like-domains 9
• multiple epidermal growth factor-like domains protein 9

Background

MEGF9 (Multiple EGF‑like domains protein 9; also EGF‑like protein 5) is a novel transmembrane glycoprotein with multiple EGF-like repeats. It is predominantly expressed in the developing and adult central nervous system and peripheral nervous system. Immunohistochemical studies showed that MEGF9 is expressed in Purkinje cells of the cerebellum and in glial cells of the peripheral nervous system. In non-neuronal tissues, MEGF9 is expressed in epidermal layer of skin, papillae of the tongue and epithelium of the gastrointestinal tract. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration.  Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular domain (ECD). The extracellular region possesses a lengthy Pro‑rich region (aa 55‑200). N-terminal region of MEGF9 includes several potential O-glycosylation sites followed by five EGF-like domains which are highly homologous to the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short intracellular domain with potential phosphorylation sites is present (1, 2). Within the ECD, human MEGF9 shares 75% aa sequence identity with mouse and rat MEGF9.

1. Brandt-Bohne, U. et al. (2007) Biochem. J. 401:447.
2. Heller, B. et al. (2014) J. Cell. Biol. 204:1219.

Bioinformatics

• Uniprot:
  • Q9H1U4.3()