Recombinant Human LRP-10 Fc Chimera Protein, CF

2 μg/lane of Recombinant Human LRP-10 Fc Chimera (Catalog # 10647-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 84-96 kDa and 160-180 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human LRP-10 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human LRPAP (Catalog # 4296-LR) is immobilized at 5 µg/mL (100 µL/well), Recombinant Human LRP-10 Fc Chimera (Catalog # 10647-LR) binds with an ED₅₀ of 12-60 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human LRP-10 protein
  

  Human LRP-10 (His17-Pro438) Accession # NP_054764.2	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: NP_054764.2
• N-terminal Sequence: His17
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 72 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 84-96 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRP-10 Fc Chimera Protein, CF

• DKFZp564C1940
• low density lipoprotein receptor-related protein 10
• low-density lipoprotein receptor-related protein 10
• LRP-10
• LRP9
• MGC142274
• MGC142276
• MGC8675
• MST087

Background

LRP-10 (Low-density lipoprotein receptor-related protein 10) is a member of the new subfamily of low-density lipoprotein receptor (LDLR) family that includes two other receptors LRP-3 and LRP-12 (1). It is called LRP-9 in the mice. This unique family is characterized by extracellular CUB domains and large cytoplasmic tails containing acidic dileucine (DXXLL) motifs (2). Mature human LRP-10 consists of a 424 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 252 aa cytoplasmic domain. The ECD contains 4 LDLR-A domains and 2 CUB domains. Within the ECD, human LRP-10 shares 90% aa sequence identity with mouse and rat LRP-10. LRP-10 is expressed in various tissues including brain and may be involved in apolipoprotein internalization (3). It is also localized in Trans‑Golgi network (TGN) and endosomes (2,4). LRP-10 is also identified as a novel sorting receptor for Amyloid precursor protein (APP) and a decrease in LRP-10 function contributes to the pathogenesis of Alzheimer's disease (5).

1. Battle, M. A. et al. (2003) Biochemistry. 42:7270.
2. Boucher, R. et al. (2008) Histochem. Cell. Biol. 130:315.
3. Sugiyama, T. et al. (2000) Biochemistry. 39:15817.
4. Doray, B. et al. (2008) Traffic. 9:1551.
5. Brodeur, J. et al. (2012) Mol. Neurodegener. 7:31.

Bioinformatics

• Uniprot:
  • NP_054764.2(Human)