Recombinant Human Layilin Protein

• Reactivity: Hu
• Applications: Binding Activity

Recombinant Human Layilin Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rhLayilin at 5 µg/mL (100 µL/well) can bind biotinylated hyaluronan with a linear range of 0.8-50 µg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Layilin protein
  Ala22-Glu220, with a C-terminal 10-His tag
• Accession #: NP_849156
• N-terminal Sequence: Ala22
• Protein/Peptide Type: Recombinant Proteins
• Gene: LAYN
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 24.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 30-35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Layilin Protein

• FLJ30977
• FLJ31092
• Layilin
• LAYN
• LYAN

Background

Layilin (named after the L-A-Y-I-L-I six amino acid motif in the molecule's transmembrane segment) is a 55 kDa member of the animal C-type lectin family. The human Layilin cDNA encodes a 374 amino acid (aa) type I transmembrane protein that, based on the orthologous molecule in hamster, contains a 19 aa signal sequence, a 201 aa extracellular region, a 31 aa transmembrane segment, and a 123 aa cytoplasmic tail (1, 2). The extracellular domain has a 130 aa carbohydrate recognition domain that contains only one potential Ca⁺⁺-binding site. The intracellular region contains three types of LH (layilin homology) repeats. There are three 16‑18 aa LH1 repeats, 3 five aa (E-G-S-F/W-V) LH2 repeats, and 2 four aa (N-D/E-I-Y) LH3 repeats. Adjacent LH2-LH3 tandem arrays provide docking sites for talin, a cytoskeleton-membrane adaptor protein (3). The human layilin extracellular region is 85% aa identical to the hamster layilin extracellular region, and 82% aa identical to the extracellular region of a similar molecule identified in mouse. Layilin is known to be a receptor for hyaluronan (HA), a negatively charged linear high molecular weight polysaccharide composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine (1, 4, 5). HA is found in the extracellular matrix of most animal tissues and in body fluids. Thus, it may be that layilin modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease. Layilin binds specifically to HA but not to other glycosaminoglycans (4).

1. Strausberg, R. (2002) GenBank Accession # AAH25407.
2. Borowsky, M.L. and R.O. Hynes (1998) J. Cell Biol. 143:429.
3. Rees, D.J.G. et al. (1990) Nature 347:685.
4. Bono, P. et al. (2001) Mol. Biol. Cell 12:891.
5. Goa, K.L. and P. Benfield (1994) Drugs 47:536.

Bioinformatics

• Gene Symbol: LAYN
• Uniprot:
  • NP_849156()