Recombinant Human Integrin alpha 9 beta 1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Integrin alpha 9 beta 1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. When 5 x 10⁴ cells per well are added to Recombinant Human Integrin alpha 9/ beta 1 coated plates (10 µg/mL, 100 µL/well), >50% will adhere after 60 minutes at 37 °C.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 9 beta 1 protein
  

  Human Integrin alpha 9 (Tyr30-Tyr977) Accession # Q13797	Acidic Tail	HHHHHH
  Human Integrin beta 1 (Gln21-Asp728) Accession # P05556	Basic Tail
  N-terminus		C-terminus

• Accession #: Q13797 (Integrin alpha 9) & P05556 (Integrin beta 1)
• N-terminal Sequence: Tyr30 ( alpha 9) & No results obtained: Gln21 predicted ( beta 1)
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGA9
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 114 kDa ( alpha 9) & 86.3 kDa ( beta 1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 130-165 kDa & 150-165 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 9 beta 1 Protein, CF

• Integrin alpha 9 beta 1

Background

Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit (1-3). It is the non-covalent heterodimer of 150 kDa alpha 9 and 130 kDa beta 1 type I transmembrane glycoprotein subunits (3). The alpha 9 extracellular domain (ECD) contains an N-terminal beta -propeller structure, followed by domains termed thigh, calf-1 and calf-2 (2). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 9 beta -propeller to form a binding domain when the dimer is in the active, extended and open conformation. The 952 amino acid (aa) human alpha 9 extracellular domain (ECD) shares 89-93% aa sequence identity with mouse, rat, canine, bovine and equine alpha 9, while the 708 aa human beta 1 ECD shares 92-96% aa sequence identity with rat, bovine, mouse, and feline beta 1. Each subunit has a transmembrane sequence and a short cytoplasmic tail which, for alpha 9, contains binding sites for paxillin and SSAT (spermine/spermidine-N-acetyltransferase) (4, 5). Integrin alpha 9 beta 1 inhibits cell spreading via signaling through paxillin (4). SSAT interaction with alpha 9 beta 1 allows catalysis that lifts spermine/spermidine inhibition of KIR potassium channels and enhances cell migration (5). Lymphangiogenic vascular endothelia growth factors VEGF-C and VEGF-D are ligands for Integrin alpha 9 beta 1 (6, 7) Genetic deletion of mouse alpha 9 beta 1 results in a fatal chylothorax due to abnormal lymphatic development (8). Integrin alpha 9 beta 1 interacts in cis with G-CSF R and enhances its signals, contributing to granulopoiesis (9). It also binds VCAM-1, which promotes neutrophil trans endothelial migration, and delays apoptosis (10, 11). Other ligands for alpha 9 beta 1 include tenascin, osteopontin, nerve growth factor, and most members of the ADAM family (12, 14).

1. Takada, Y. et al. (2007) Genome Biol. 8:215. 
2. Luo, B-H. et al. (2007) Annu. Rev. Immunol. 25:619.
3. Palmer, E. L. et al. (1993) J. Cell Biol. 123:1289.
4. Liu, S. et al. (2001) J. Biol. Chem. 276:37086.
5. deHart, G. W. et al. (2008) Proc. Natl. Acad. Sci. USA 105:7188.
6. Vlahakis, N. E. et al. (2005) J. Biol. Chem. 280:4544.
7. Vlahakis, N. E. et al. (2007) J. Biol. Chem. 282:15187.
8. Huang, X. Z. et al. (2000) Mol. Cell. Biol. 20:5208.
9. Chen, C. et al. (2006) Immunity 25:895.
10. Ross, E. A. et al. (2006) Blood 107:1178.
11. Taooka, Y. et al. (1999) J. Cell Biol. 145:413.
12. Marcinkiewicz, C. et al. (2000) J. Biol. Chem. 275:31930.
13. Staniszewska, I. et al. (2008) J. Cell Sci. 121:504.
14. Eto, K. et al. (2002) J. Biol. Chem. 277:17804.

Publications for Integrin alpha 9 beta 1 (5438-A9)(1)

Publication using 5438-A9

• G Fnu, P Agrawal, GC Kundu, GF Weber Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44 Biomolecules, 2021-05-30;11(6):. 2021-05-30 [PMID: 34070790] (SPR, Human)

Bioinformatics

• Gene Symbol: ITGA9
• Uniprot:
  • Q13797 (Integrin alpha 9) & P05556 (Integrin beta 1)()