Recombinant Human IL-10 R beta Fc Chimera Protein, CF

Images

 
There are currently no images for IL-10 R beta (874-RB).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human IL-10 R beta Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human IL‑28 R alpha /IFN‑ lambda  R1 Fc Chimera (Catalog # 5260-MR) is immobilized at 0.5 μg/well, the concentration of Recombinant Human IL-10 R beta Fc Chimera that produces 50% of the optimal binding response is found to be approximately 4-16 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human IL-10 R beta protein
Human IL-10 R beta
(Met20-Ser220)
Accession # Q08334
DIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Met20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
IL10RB
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
51 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-85 kDa, reducing conditions
Publications
Read Publication using
874-RB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-10 R beta Fc Chimera Protein, CF

  • CDw210b antigen
  • CDw210b
  • CRF2-4IL-10R subunit 2
  • CRFB4
  • CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B
  • D21S58
  • D21S66
  • IBD25
  • IL-10 R beta
  • IL-10 receptor subunit beta
  • IL10R beta
  • IL-10R2
  • IL-10R2IL-10R subunit beta
  • IL10RB
  • IL-10Rb
  • interleukin 10 receptor, beta
  • Interleukin-10 receptor subunit 2
  • interleukin-10 receptor subunit beta
  • member 4

Background

Interleukin-10 Receptor beta (IL-10 R beta ), also known as IL-10 R2 and CRF2-4, is a 60 kDa transmembrane glycoprotein that functions as a co-receptor for several class 2 cytokines including Interleukins-10, -22, -26, -28A/IFN-lambda 2, -28B/IFN-lambda 3, and -29/IFN-lambda (1, 2). IL-10 R beta associates with ligand‑specific receptor subunits to form signaling receptor complexes, e.g. IL-10 R alpha for IL-10 (3, 4), IL-20 R alpha for IL-26 (5, 6), IL-22 R alpha for IL-22 (7, 8), and IL-28 R alpha for IL-28A, IL-28B, and IL-29 (9, 10). IL‑10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligand‑specific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL-10 R beta deficient mice lack responsiveness to each of those cytokines. IL-10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligand‑specific subunit (8, 9, 11). In the case of IL-10, a cytokine dimer binds to two IL‑10 R alpha /IL-10R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains (3, 12). Some members of the IL-10 family are monomeric cytokines and interact with single molecules of IL-10 R beta and their ligand‑specific subunit (1). Mature human IL-10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin type-III domains, a 22 aa transmembrane segment and a 83 aa cytoplasmic domain (13). Within the ECD, human IL-10 R beta shares 75% and 78% aa sequence identity with mouse and rat IL-10 R beta, respectively.
  1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  3. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  4. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  5. Sheikh, F. et al. (2004) J. Immunol. 172:2006.
  6. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  7. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  8. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  9. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  10. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  11. Yoon, S.I. et al. (2006) J. Biol. Chem. 281:35088.
  12. Pletnev, S. et al. (2005) BMC Struct. Biol. 5:10.
  13. Lutfalla, G. et al. (1993) Genomics 16:366.

Customers Who Viewed This Item Also Viewed...

MAB15041
Species: Hu
Applications: WB, IHC, CyTOF-ready, ICFlow
MAB274
Species: Hu
Applications: Neut
AF1375
Species: Hu
Applications: WB
NBP2-22471
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
AF1709
Species: Mu
Applications: WB, Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ICFlow, ELISA(Sta)
1102-IL/CF
Species: Hu
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
NBP2-27210
Species: Hu, Fe, Pm
Applications: WB, IHC, IHC-P
NBP2-67339
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, IP
NB100-56409
Species: Hu, Mu
Applications: WB, Flow

Publications for IL-10 R beta (874-RB)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


Filter By Application
Bioassay
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for IL-10 R beta (874-RB) (0)

There are no reviews for IL-10 R beta (874-RB). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-10 R beta (874-RB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-10 R beta Products

Bioinformatics Tool for IL-10 R beta (874-RB)

Discover related pathways, diseases and genes to IL-10 R beta (874-RB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-10 R beta (874-RB)

Discover more about diseases related to IL-10 R beta (874-RB).
 

Pathways for IL-10 R beta (874-RB)

View related products by pathway.

PTMs for IL-10 R beta (874-RB)

Learn more about PTMs related to IL-10 R beta (874-RB).

Blogs on IL-10 R beta

There are no specific blogs for IL-10 R beta, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human IL-10 R beta Fc Chimera Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol IL10RB
Uniprot