Recombinant Human IL-10 R beta Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-10 R beta Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human IL 28 R alpha /IFN lambda R1 Fc Chimera (Catalog # 5260-MR) is immobilized at 5.00 μg/mL (100 μL/well), the concentration of Recombinant Human IL-10 R beta Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.300-3.00 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human IL-10 R beta protein
  

  Human IL-10 R beta (Met20-Ser220) Accession # Q08334	DIEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: Q08334
• N-terminal Sequence: Met20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL10RB
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 51 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 75-85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-10 R beta Fc Chimera Protein, CF

• CDw210b antigen
• CDw210b
• CRF2-4IL-10R subunit 2
• CRFB4
• CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B
• D21S58
• D21S66
• IBD25
• IL-10 R beta
• IL-10 receptor subunit beta
• IL10R beta
• IL-10R2
• IL-10R2IL-10R subunit beta
• IL10RB
• IL-10Rb
• interleukin 10 receptor, beta
• Interleukin-10 receptor subunit 2
• interleukin-10 receptor subunit beta
• member 4

Background

Interleukin-10 Receptor beta (IL-10 R beta ), also known as IL-10 R2 and CRF2-4, is a 60 kDa transmembrane glycoprotein that functions as a co-receptor for several class 2 cytokines including Interleukins-10, -22, -26, -28A/IFN-lambda 2, -28B/IFN-lambda 3, and -29/IFN-lambda (1, 2). IL-10 R beta associates with ligand‑specific receptor subunits to form signaling receptor complexes, e.g. IL-10 R alpha for IL-10 (3, 4), IL-20 R alpha for IL-26 (5, 6), IL-22 R alpha for IL-22 (7, 8), and IL-28 R alpha for IL-28A, IL-28B, and IL-29 (9, 10). IL‑10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligand‑specific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL-10 R beta deficient mice lack responsiveness to each of those cytokines. IL-10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligand‑specific subunit (8, 9, 11). In the case of IL-10, a cytokine dimer binds to two IL‑10 R alpha /IL-10R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains (3, 12). Some members of the IL-10 family are monomeric cytokines and interact with single molecules of IL-10 R beta and their ligand‑specific subunit (1). Mature human IL-10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin type-III domains, a 22 aa transmembrane segment and a 83 aa cytoplasmic domain (13). Within the ECD, human IL-10 R beta shares 75% and 78% aa sequence identity with mouse and rat IL-10 R beta , respectively.

1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
3. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
4. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
5. Sheikh, F. et al. (2004) J. Immunol. 172:2006.
6. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
7. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
8. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
9. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
10. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
11. Yoon, S.I. et al. (2006) J. Biol. Chem. 281:35088.
12. Pletnev, S. et al. (2005) BMC Struct. Biol. 5:10.
13. Lutfalla, G. et al. (1993) Genomics 16:366.

Publications for IL-10 R beta (874-RB)(2)

Publications using 874-RB

• DM Santer, GES Minty, DP Golec, J Lu, J May, A Namdar, J Shah, S Elahi, D Proud, M Joyce, DL Tyrrell, M Houghton Differential expression of interferon-lambda receptor 1 splice variants determines the magnitude of the antiviral response induced by interferon-lambda 3 in human immune cells PLoS Pathog., 2020-04-30;16(4):e1008515. 2020-04-30 [PMID: 32353085] (Bioassay, Human)
• Donnelly RP, Sheikh F, Kotenko SV, Dickensheets H The expanded family of class II cytokines that share the IL-10 receptor-2 (IL-10R2) chain. J. Leukoc. Biol., 2004-05-03;76(2):314-21. 2004-05-03 [PMID: 15123776] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL10RB
• Uniprot:
  • Q08334()