Recombinant Human IGSF4D/CADM2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IGSF4D/CADM2 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 50 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human IGSF4D/CADM2 protein

  Human IGSF4D/CADM2 (Ala21-Asp335) Accession # AAI06000

  6-His tag

  N-terminus

  C-terminus

• Accession #: AAI06000
• N-terminal Sequence: Ala21
• Protein/Peptide Type: Recombinant Proteins
• Gene: CADM2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35.5 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 36-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGSF4D/CADM2 Protein, CF

• CADM2
• cell adhesion molecule 2
• IGSF4D
• IGSF4DMGC138343
• Immunoglobulin superfamily member 4D
• immunoglobulin superfamily, member 4D
• MGC104534
• Necl-3
• NECL3MGC138341
• nectin-like 3
• Nectin-like protein 3
• SynCAM2

Background

IGSF4D is an immunoglobulin superfamily member that is also designated as synaptic cell adhesion molecule 2 (SynCAM 2) and nectin-like protein 3 (Necl-3) (1). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in epithelial cells that contain one V-type Ig-like and two C2-type Ig-like domains in their extracellular regions. These domains are characteristically responsible for Ca ++ -independent homophilic and heterophilic interactions in the brain, lung, kidney, bladder, prostate and testis (1-3). Based on homology with other family members, the 437 amino acid (aa) human IGSF4D contains a 355 aa extracellular domain (ECD) that shares > 99% aa identity with corresponding regions of canine and bovine, and 93% aa identity with rat and mouse IGSF4D. Rat and mouse IGSF4D lack an exon at the end of the putative ECD. If this exon is disregarded, aa identity with human IGSF4D rises to > 98%. IGSF4A, B, C and D proteins share 35-50% aa identity within the ECD. Amino acids found to be critical in other IGSF4 proteins for adhesion and intracellular binding of MAGUK guanylate kinase subfamily proteins are conserved in IGSF4D (3). The expression and function of IGSF4D has not been well characterized. However, in-house quality control data at R&D Systems has shown that, like other family members, IGSF4D has a positive effect on in vitro outgrowth of cortical neurons.

1. Biederer, T. et al. (2006) Genomics 87:139.
2. Sakisaka T. and Y. Takai (2004) Curr. Opin. Cell Biol. 16:513.
3. Kakunaga, S. et al. (2004) J. Cell Sci. 118:1267.

Bioinformatics

• Gene Symbol: CADM2
• Uniprot:
  • AAI06000()