Recombinant Human IFN-epsilon Protein

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Recombinant Human IFN-epsilon (Catalog # 9667-ME) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 100‑500 ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IFN-epsilon Protein Summary

Details of Functionality
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 100-500 ng/mL.
Source
E. coli-derived human IFN-epsilon protein
Leu22-Arg208 (Cys175Ser), with an N-terminal Met and a C-terminal 6-His tag
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
23 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 250 μg/mL in water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-epsilon Protein

  • IFNE
  • IFN-E
  • IFNE1PRO655
  • IFNepsilon
  • IFN-epsilon
  • IFNT1
  • Ifn-tau-11
  • INFE1
  • interferon epsilon 1
  • interferon epsilon
  • Interferon epsilon-1
  • interferon tau-1
  • interferon, epsilon
  • interferon-epsilon
  • MGC119018
  • MGC119020

Background

Interferon-epsilon (IFN-epsilon) is a secreted, approximately 23 kDa member of the type I interferon family of molecules (1). Mature human IFN-epsilon shares 62% and 60% amino acid sequence identity with rat and mouse IFN-epsilon, respectively. IFN-epsilon signals through IFN‑alpha / beta R1 and IFN‑alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further up-regulated in the uterus by estrogen (2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation, attraction of CD8+ T cells to sites of virus infection and directly mediates protection against viral and bacterial genital infections (5, 7). Differential regulation of genes related to central nervous system by recombinant human IFN-epsilon suggests its role in maintenance of the structure and function of brain (8).
  1. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
  2. Fung, K.Y. et al. (2013) Science 339:1088.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
  5. Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
  6. Hermant, P. et al. (2013) PLoS One 8:e71320.
  7. Day, S.L. et al. (2008) J. Immunol. 180:7158.
  8. Peng, Fu-Wang et.al. (2007) Protein Expression and Purification 53:356.

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