Recombinant Human His6-ZRANB1/Trabid Protein, CF

Product Discontinued

Recombinant Human His6-ZRANB1/Trabid Protein, CF Summary

• Details of Functionality: Recombinant Human His6-ZRANB1/Trabid is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-ZRANB1/Trabid concentration of 0.1-1 μM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ZRANB1/Trabid protein
  Contains a C-terminal 6-His tag
• Accession #: Q9UGI0
• Protein/Peptide Type: Recombinant Enzymes
• Gene: ZRANB1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer:

  X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 1 mM TCEP

• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-ZRANB1/Trabid Protein, CF

• HRPE773
• jacalin-like lectin domain containing 2
• JCLN2
• pancreatic adenocarcinoma upregulated factor
• PAUF
• PRO1567
• Trabid
• ZRANB1
• zymogen granule protein 16 homolog B (rat)
• zymogen granule protein 16 homolog B

Background

Zinc Finger, RAN-binding Domain Containing 1 (ZRANB1), also known as TRAF-binding Domain-containing Protein (Trabid), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 81 kDa (1). The human protein shares 99% amino acid sequence identity with its mouse ortholog. ZRANB1 preferentially cleaves K29-, K33-, and K63-linked poly-Ubiquitin chains (2). It has been shown to play a role in the regulation of Wnt signaling via deubiquitination of APC (3,4). This recombinant protein contains a C-terminal 6-His tag.

1. Evans, P.C. et al. (2001) Biochem. J. 357:617.
2. Licchesi, J.D. et al. (2011) Nat. Struct. Mol. Biol. 19:62.
3. Tran, H. et al. (2008) Genes Dev. 22:528.
4. Shi, T. et al. (2012) BMC Chem. Biol. 12:4.
 

Publications for ZRANB1/Trabid (E-560)(2)

Publications using E-560

• Chen, YH;Chen, HH;Wang, WJ;Chen, HY;Huang, WS;Kao, CH;Lee, SR;Yeat, NY;Yan, RL;Chan, SJ;Wu, KP;Chen, RH; TRABID inhibition activates cGAS/STING-mediated anti-tumor immunity through mitosis and autophagy dysregulation Nature communications 2023-05-26 [PMID: 37237031] (Bioassay, N/A)
• YH Chen, TY Huang, YT Lin, SY Lin, WH Li, HJ Hsiao, RL Yan, HW Tang, ZQ Shen, GC Chen, KP Wu, TF Tsai, RH Chen VPS34 K29/K48 branched ubiquitination governed by UBE3C and TRABID regulates autophagy, proteostasis and liver metabolism Nature Communications, 2021-02-26;12(1):1322. 2021-02-26 [PMID: 33637724] (Ubiquitination, Human)

Bioinformatics

• Gene Symbol: ZRANB1
• Uniprot:
  • Q9UGI0(Human)