Recombinant Human His6-VCIP135/VCPIP1 Protein, CF

Product Discontinued

Recombinant Human His6-VCIP135/VCPIP1 Protein, CF Summary

• Details of Functionality:

  Reaction conditions will need to be optimized for each specific application. We recommend an initial VCIP135 concentration of 100-500 nM in reactions utilizing Ubiquitin-AMC or Ubiquitin-Rhodamine substrates (U-550 or U-555) or Poly-ubiquitin chain substrates.

• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VCIP135/VCPIP1 protein
  Contains an C-terminal 6-His tag
• Accession #: Q96JH7
• Protein/Peptide Type: Recombinant Enzymes
• Gene: VCPIP1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 135 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-VCIP135/VCPIP1 Protein, CF

• DKFZp686G038
• DUBA3
• EC 3.4.22.-
• FLJ23132
• KIAA1850FLJ60694
• valosin containing protein (p97)/p47 complex interacting protein 1
• valosin-containing protein (p97)/p47 complex-interacting protein p135
• Valosin-containing protein p97/p47 complex-interacting protein 1
• Valosin-containing protein p97/p47 complex-interacting protein p135
• VCIP135
• VCIP135deubiquitinating protein VCIP135
• VCPIP1 VCIP135
• VCPIP1

Background

Valosin-containing protein p97/p47 complex-interacting protein, or VCIP135, is a member of the OTU superfamily of Deubiquitinating Enzymes. It is 1222 amino acids (aa) in length with a predicted molecular weight of 134 kDa. Human VCIP135 shares 95% aa sequence identity with the mouse ortholog. In the cell, VCIP135 function requires membrane association and interaction with p97, both of which are inhibited by VCIP135 phosphorylation during mitosis. VCIP135 activity is necessary for VCP-mediated reassembly of Golgi stacks after mitosis. In vitro, VCIP135 efficiently hydrolyzes K11- and K48-linked Poly-ubiquitin chains.

1. Zhang, X. et al.  (2014) Cell Sci  127: 172-181

Bioinformatics

• Gene Symbol: VCPIP1
• Uniprot:
  • Q96JH7()