Recombinant Human His6-NEDD4 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human His6-NEDD4 Protein, CF Summary

• Details of Functionality: Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application.
• Source: Trichoplusia ni, T. ni (baculovirus)-derived human NEDD4 protein
  Leu570 - Asp1319 with a N-terminal Met and 6-His tag
• Accession #: P46934.4
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 88 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT and EDTA.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-NEDD4 Protein, CF

• Cell proliferation-inducing gene 53 protein
• E3 ubiquitin-protein ligase NEDD4
• EC 6.3.2
• EC 6.3.2.-
• KIAA0093NEDD4-1MGC176705
• NEDD4
• NEDD-4
• Neural precursor cell expressed developmentally down-regulated protein 4
• neural precursor cell expressed, developmentally down-regulated 4
• receptor-potentiating factor 1
• RPF1

Background

NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) is a Ubiquitin E3 ligase and the prototypical member of an extended family of HECT domain ligases.  Human family members include NEDD4, NEDD4-2, ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDDL1 and NEDDL2.  In addition to the HECT domain, NEDD4 family members also contain a C2 domain and one or more WW domains.  With a predicted molecular weight of 150 kDa, NEDD4 is expressed in most human tissues where it localizes primarily to the perinuclear and other membrane regions.  Evidence suggests that NEDD4 down-regulates multiple membrane proteins (including FGFR1, IGFR1, and multiple EGFR family members) in a Ubiquitin-dependent internalization and lysosomal degradation process.  NEDD4 also plays a role in facilitating the sequestration of alpha-synuclein to cellular endosomes.  

1. Harvey, K.F. and S. Kumar (1999) Trends Cell Biol. 9:166.
2. Maspero, E., et al. (2013) Nat. Struct. Mol. Biol. 20:696.
3. Scheffner, M. and S. Kumar (2014) Biochim. Biophys. Acta. 1843:61.
4. Sugeno, N., et al. (2014) J. Biol. Chem. 289:18137.
5. Yang, B. and S. Kumar (2010) Cell Death Differ. 17:68.

Publications for NEDD4 (E3-290)(1)

Publication using E3-290

• Zenko, D;Marsh, J;Castle, AR;Lewin, R;Fischer, R;Tofaris, GK; Monitoring ?-synuclein ubiquitination dynamics reveals key endosomal effectors mediating its trafficking and degradation Science advances 2023-06-16 [PMID: 37315142] (Bioassay, Human)

Bioinformatics

• Uniprot:
  • P46934.4()