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![Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Western-Blot-NBP2-27197-img0001.jpg "Western Blot: NEDD4 Antibody [NBP2-27197] - analysis of NEDD4 in mouse embryonic brain tissue lysate in the 1) absence and 2) presence of immunizing peptide using NEDD4 antibody at 5 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32641_rabbit-nedd4-mab-je35-58-278202416331277.jpg "Western Blot: NEDD4 Antibody (JE35-58) [NBP3-32641] - Western blot analysis of NEDD4 on different lysates with Rabbit anti-NEDD4 antibody (NBP3-32641) at 1/2,000 dilution. Lane 1: U87MG cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 149 kDa Observed band size: 110 kDa Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32641) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Western Blot:[NBP3-30524]-Detection of human NEDD4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat and HEK293T cells. Antibodies: Affinity purified rabbit anti-NEDD4 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. NEDD4 was also immunoprecipitated by rabbit anti-NEDD4 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30524_rabbit-nedd4-pab-23120251204059.jpg "Western Blot:[NBP3-30524]-Detection of human NEDD4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , Jurkat and HEK293T cells. Antibodies: Affinity purified rabbit anti-NEDD4 antibody used for WB at 0. 1 µg/ml and 1 µg/ml and used for IP at 6 µg/mg lysate. NEDD4 was also immunoprecipitated by rabbit anti-NEDD4 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds.")
![Western Blot: NEDD4 Antibody (7R0M4) [NBP3-16398] - Western blot analysis of extracts of various cell lines, using NEDD4 Rabbit mAb (NBP3-16398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/NEDD4-Antibody-7R0M4-Western-Blot-NBP3-16398-img0001.jpg "Western Blot: NEDD4 Antibody (7R0M4) [NBP3-16398] - Western blot analysis of extracts of various cell lines, using NEDD4 Rabbit mAb (NBP3-16398) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: NEDD4 Antibody [NBP2-97422] - Immunochemical staining of human NEDD4 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/NEDD4-Antibody-Immunohistochemistry-Paraffin-NBP2-97422-img0001.jpg "Immunohistochemistry-Paraffin: NEDD4 Antibody [NBP2-97422] - Immunochemical staining of human NEDD4 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.")
![Immunoprecipitation:NEDD4 AntibodyNBP3-29418] -Detection of human NEDD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEDD4 antibody NBP3-29418 (lot NBP3-29418-2) used for IP at 6 µg per reaction. NEDD4 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29418-1) and rabbit anti-NEDD4.. For blotting immunoprecipitated NEDD4, . was used at 1.0 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-29418_rabbit-nedd4-pab-301220241518243.jpg "Immunoprecipitation:NEDD4 AntibodyNBP3-29418] -Detection of human NEDD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NEDD4 antibody NBP3-29418 (lot NBP3-29418-2) used for IP at 6 µg per reaction. NEDD4 was also immunoprecipitated by a previous lot of this antibody (lot NBP3-29418-1) and rabbit anti-NEDD4.. For blotting immunoprecipitated NEDD4, . was used at 1.0 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2. Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/NEDD4L-Antibody-1D2-Western-Blot-H00023327-M04-img0003.jpg "Western Blot: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L expression in transfected 293T cell line by NEDD4L monoclonal antibody (M04), clone 1D2. Lane 1: NEDD4L transfected lysate (Predicted MW: 104.9 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation: NEDD4L Antibody (1D2) [H00023327-M04] - Analysis of NEDD4L transfected lysate using anti-NEDD4L monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEDD4L monoclonal antibody.](http://images.novusbio.com/fullsize/NEDD4L-Antibody-1D2-Immunoprecipitation-H00023327-M04-img0001.jpg)
![Sandwich ELISA: NEDD4L Antibody (1D2) [H00023327-M04] - Detection limit for recombinant GST tagged NEDD4L is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NEDD4L-Antibody-1D2-Sandwich-ELISA-H00023327-M04-img0002.jpg)
![Immunohistochemistry:[NBP3-30204]-Detection of human NEDD4L by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NEDD4L used at a dilution of 1: 200. Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30204_rabbit-nedd4l-pab-23120251252844.jpg "Immunohistochemistry:[NBP3-30204]-Detection of human NEDD4L by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NEDD4L used at a dilution of 1: 200. Detection: DAB")
![Immunohistochemistry:[NBP3-29257]-Detection of mouse NEDD4L by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-NEDD4L.](http://images.novusbio.com/fullsize/nbp3-29257_rabbit-nedd4l-pab-231202512101616.jpg "Immunohistochemistry:[NBP3-29257]-Detection of mouse NEDD4L by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-NEDD4L.")