Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002). The IC50 value is <4.6 nM, as measured under the described conditions.
Mouse myeloma cell line, NS0-derived human HAI-2A protein Met1-Lys197, with a C-terminal 10-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
21 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Human HAI-2A Protein, CF
Two alternatively spliced forms of HAI-2 have been found in human tissues (1). HAI-2A, the full-length molecule and also known as placental bikunin, is a major form expressed in human tissues. Encoded by the SPINT2 gene, HAI-2A consists of two Kunitz domains, and a C-terminal transmembrane domain (1‑5). Both Kunitz domains can function as inhibitors independent of each other. In addition to HGF activator and trysin, HAI-2A strongly inhibits plasmin, tissue and plasma kallikreins, and factor XIa. In comparison, HAI-2A is a weaker inhibitor of factor VIIa-tissue factor, factors IXa, Xa, and XIIa. Recombinant HAI-2A prolonged the clotting time in an activated partial thromboplastin time assay.
Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
Kawaguchi, T. et al. (1997) J. Biol. Chem. 272:27558.
Marlor. C.W. et al. (1997) J. Biol. Chem. 272:12202.
Muller-Pillasch, F. et al. (1998) Biochim. Biophys. Acta 1395:88.
Delaria, K.A. et al. (1997) J. Biol. Chem. 272:12209.
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