Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

2 μg/lane of Recombinant Human Guanylyl Cyclase C/GUCY2C was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 73-81 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Guanylyl Cyclase C His-tag Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
  Ser21-Gln430, with a C-terminal 6-His tag
• Accession #: P25092.2
• N-terminal Sequence: Ser21
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 73-81 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

• DIAR6
• EC 4.6.1
• GCC
• GC-C
• guanylate cyclase 2C (heat stable enterotoxin receptor)
• Guanylate Cyclase C
• Guanylyl Cyclase C
• GUC2C
• GUC2CEC 4.6.1.2
• GUCY2C
• heat-stable enterotoxin receptor
• hSTAR
• Intestinal guanylate cyclase
• MUCIL
• STA Receptor
• StAR
• STARSTA receptor

Background

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
7. Gong, R. et al. (2011) Science. 333:1642.
8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Bioinformatics

• Uniprot:
  • P25092.2()