Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF

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When Human Guanylyl Cyclase C Antibody (MAB2157) is immobilized at 100 ng/mL (100 µL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag (Catalog # AVI2157) binds with an ED50 of 0.250-1.50 ng/mL
2 μg/lane of Biotinylated Recombinant Human Guanylyl Cyclase C/GUCY2C His-tag Avi-tag Protein (Catalog # AVI2157) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF Summary

Additional Information
Biotinylated /GUCY2C His-tag
Details of Functionality
Measured by its binding ability in a functional ELISA. When Human Guanylyl Cyclase C Antibody (Catalog # MAB2157) is immobilized at 100 ng/mL (100 µL/well), Biotinylated Recombinant Human Guanylyl Cyclase C His-tag Avi-tag binds with an ED50 of 0.250-1.50 ng/mL. Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons. 2.50 μg/mL of protein is able to significantly inhibit neurite outgrowth.
Source
Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
Human Guanylyl Cyclase C Protein
(Ser24-Gln430)
Accession # P25092.2
6-His tagAvi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Ser24
Structure / Form
Biotinylated via Avi-tag
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-85 kDa, under reducing conditions.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 1.00 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Guanylyl Cyclase C His Avi-tag Protein, CF

  • DIAR6
  • EC 4.6.1
  • GCC
  • GC-C
  • guanylate cyclase 2C (heat stable enterotoxin receptor)
  • Guanylate Cyclase C
  • Guanylyl Cyclase C
  • GUC2C
  • GUC2CEC 4.6.1.2
  • GUCY2C
  • heat-stable enterotoxin receptor
  • hSTAR
  • Intestinal guanylate cyclase
  • MUCIL
  • STA Receptor
  • StAR
  • STARSTA receptor

Background

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family and helps regulate intestinal function (1, 8). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (1 - 4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a pseudokinase domain and a GC catalytic domain. The ECD of mature human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. GUCY2C is primarily expressed in intestinal epithelial cells from the duodenum to rectum and binds to the endogenous gastrointestinal hormones guanylin and uroguanylin (1-4). Activation of GUCY2C signaling mediates fluid-ion homeostasis, intestinal inflammation, and cell proliferation in a cGMP-dependent manner (5). GUCY2C is also expressed in neurons, where it plays a role in attention deficiency/hyperactive behavior (6). GUCY2C is being investigated as a treatment for several gastrointestinal disorders including irritable bowel syndrome, obesity, colorectal cancer, and chronic idiopathic constipation (7, 8). Our Avi-tag Biotinylated GUCY2C features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
  1. Arshad, N. and Visweswariah S.S. (2012) FEBS Lett. 586:2835.
  2. Potter, L.R. (2011) Cell Signal (2011) 23:1921.
  3. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  4. Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
  5. Lucas, K. et al. (2000) Pharmacol Rev. 52:375.
  6. Gong, R. et al. (2011) Science 333:1642.
  7. Waldman, S.A and Camilleri, M. (2018) Gut 67:1543.
  8. Kim, G.W. et al. (2013) Trends Endocrinol Metab 24(4):165.

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