Recombinant Human GPR158 His-tag Protein, CF

When Recombinant Human GPR158 (Catalog # 10286-GP) is coated at 1 μg/mL, 100 μL/well, Recombinant Human Neuropilin-1 (Catalog # 3870-N1) binds with an ED50 of 0.8‑4 μg/mL.

2 μg/lane of Recombinant Human GPR158 His-tag Protein (Catalog # 10286-GP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-70 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human GPR158 His-tag Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human GPR158 is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neuropilin-1 (Catalog # 3870-N1) with an ED₅₀ of 0.8-4.8 μg/mL
• Source: Human embryonic kidney cell, HEK293-derived human GPR158 protein
  Ala24-Gln411, with a C-terminal 6-His tag
• Accession #: Q5T848
• N-terminal Sequence: Ala24 & Ser25
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-70 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GPR158 His-tag Protein, CF

• G protein-coupled receptor 158
• GPR158
• KIAA1136
• KIAA1136FLJ37801
• probable G-protein coupled receptor 158
• RP11-59G22.1

Background

G-protein coupled receptor 158 (GPR158) is a receptor belonging to the Class C GPCR family. It lacks the extracellular Venus flytrap module characteristic of the known members of that family and instead contains two other elements that are not typical of the class: a calcium-binding EGF-like domain and a leucine repeat region (1, 2). The mature extracellular domain of human GPR158 contains 393 amino acids (aa) and shares 89% identity with both mouse and rat GPR158. GPR158 is expressed at the highest level in the brain, but also in a variety of other tissues including retina, spleen, liver and lung (3). GPR158 was originally identified in functional screens linked with biological stress and has been implicated in the osteocalcin effect on cognitive processes in the brain (4, 5), and glaucoma and cancer in the periphery (4, 6).

1. Jingami, H. et al. (2003) Curr. Opin. Neurobiol. 13:271.
2. Bjarnadóttir, T.K et al. (2005) Gene. 362:70.
3. Orlandi, C. et al. (2012) J. Cell Biol. 197:711.
4. Itakura, T. et al. (2019) J. Ocul. Pharmacol. Ther. 35:203.
5. Khrimian, L. et al. (2017) J. Exp. Med. 214:2859.
6. Fenner, A. (2015) Nat. Rev. Urol. 12:182.

Bioinformatics

• Uniprot:
  • Q5T848()