Recombinant Human GM-CSF R alpha Protein, CF

• Reactivity: Hu
• Applications: Inhibition Activity
• Format: Carrier-Free

Recombinant Human GM-CSF R alpha Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit GM-CSF-dependent proliferation of TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 1.25-5 μg/mL in the presence of 40 pg/mL of rhGM-CSF.
• Source: Mouse myeloma cell line, NS0-derived human GM-CSF R alpha protein
  Leu20-Gly320, with a C-terminal 10-His tag
• Accession #: P15509
• N-terminal Sequence: Leu20
• Protein/Peptide Type: Recombinant Proteins
• Gene: CSF2RA
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Inhibition Activity
• Theoretical MW: 36.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GM-CSF R alpha Protein, CF

• CD116 antigen
• CD116
• CDw116
• colony stimulating factor 2 receptor alpha subunit
• colony stimulating factor 2 receptor, alpha, low-affinity(granulocyte-macrophage)
• CSF2R
• CSF2RA
• CSF2RAX
• CSF2RAY
• CSF2RX
• CSF2RY
• GM-CSF R alpha
• GM-CSF receptor alpha subunit
• GMCSFR alpha
• GMCSFR
• GM-CSFRa
• GM-CSF-R-alpha
• GMCSFR-alpha
• GMR
• GMR-alpha
• granulocyte-macrophage colony-stimulating factor receptor alpha chain
• granulocyte-macrophage colony-stimulating factor receptor subunit alpha
• MGC3848
• MGC4838

Background

Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1 - 4). Mature human GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 26 aa transmembrane segment, and a 54 aa cytoplasmic domain (5). Within the ECD, human GM-CSF R alpha shares approximately 33% aa sequence identity with mouse and rat GM-CSF R alpha . Alternate splicing of human GM-CSF R alpha generates several additional isoforms that lack the cytoplasmic and/or transmembrane regions. Soluble forms of the receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non-hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta _c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta _c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9 - 11). The shared usage of beta _c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
2. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
5. Gearing, D.P. et al. (1989) EMBO J. 8:3667.
6. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
7. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
8. Chiba, S. et al. (1990) Cell Regul. 1:327.
9. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
10. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
11. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.

Publications for GM-CSFR alpha (706-GR)(1)

Publication using 706-GR

• Vatter H, Brinton M Differential responses of disease-resistant and disease-susceptible primate macrophages and myeloid dendritic cells to simian hemorrhagic fever virus infection. J Virol, 2013-12-11;88(4):2095-106. 2013-12-11 [PMID: 24335289] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio anubis (Olive Baboon))

Bioinformatics

• Gene Symbol: CSF2RA
• Uniprot:
  • P15509()