Recombinant Human Frizzled-1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Frizzled-1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. In a 100 µL reaction mixture containing biotinylated rmWnt-5a at 100 ng/mL and rhFrizzled-1/Fc Chimera dilutions at 0.1-8,000 ng/mL, the concentration of rhFrizzled-1/Fc Chimera that produces 50% of the optimal binding response is found to be approximately 20-80 ng/mL.
Optimal concentrations should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human Frizzled-1 protein
Human Frizzled-1
(Gln73 - His253)
Accession # Q9UP38
IEGRD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Gly82 & No results obtained: Gln73 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
FZD1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
46.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
59-66 kDa, reducing conditions
Publications
Read Publications using
5988-FZ in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 300 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Frizzled-1 Fc Chimera Protein, CF

  • DKFZp564G072
  • FLJ95923
  • frizzled family receptor 1
  • frizzled homolog 1
  • frizzled, Drosophila, homolog of, 1
  • Frizzled1
  • Frizzled-1
  • fz-1
  • FZD1
  • fzE1
  • hFz1
  • seven transmembrane spanning receptor
  • Wnt receptor

Background

Frizzled-1 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1 - 3). Frizzled proteins are thought to be G-protein-coupled (1, 4). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Frizzled-1 can also act as a Wnt antagonist, for example, when sequestered from LRP-5/6 by LRP-1 (5, 6). Human Frizzled-1 cDNA encodes 647 aa including a 69 aa signal peptide, an extracellular cysteine-rich domain (CRD, aa 111 - 230) that binds Wnts and is highly conserved among Frizzleds, a linker region, the 7TM region, and the C-terminal cytoplasmic domain (2). Most Frizzleds, including Frizzled-1, contain a C-terminal PDZ binding motif (1). Within aa 73 - 253, human Frizzled-1 shares 94%, 93%, 97% and 98% aa identity with mouse, rat, bovine and canine Frizzled-1, respectively. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 7). Frizzled-1 shares high aa identity (~76%) with Frizzled-2 and -7, but may differ in expression and Wnt selectivity (3, 8). Specifically, Wnt-3a interaction with rat Frizzled-1, but not Frizzled-2, blocks toxicity of the Alzheimers A beta peptide on PC12 cells (8). In bone, Frizzled-1 may be part of a feedback mechanism to modulate the effects of BMP-2 on mesenchymal cells (6, 9). Frizzled-1 is also one of the purported Wnt10b receptors whose signaling inhibits adipocyte differentiation (10). It is widely expressed, with mRNA detection in adult heart, placenta, lung, kidney, pancreas, prostate, and ovary, and in fetal lung and kidney (2).

  1. Schulte, G. and B. Vitezslav (2007) Trends Pharmacol. Sci. 28:518.
  2. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  3. Gazit, A. et al. (1999) Oncogene 18:5959.
  4. Liu, T. et al. (1999) J. Biol. Chem. 274:33539.
  5. Zilberberg, A. et al. (2004) J. Biol. Chem. 279:17535.
  6. Roman-Roman, S. et al. (2004) J. Biol. Chem. 279:5725.
  7. Kaykas, A. et al. (2004) Nat. Cell Biol. 6:52.
  8. Chacon, M.A. et al. (2008) J. Cell. Physiol. 217:215.
  9. Yerges, L.M. et al. (2009) J. Bone Miner. Res. 24:87.
  10. Bennett, C.N. et al. (2002) J. Biol. Chem. 277:30998.

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Publications for Frizzled-1 (5988-FZ)(2)

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Bioinformatics

Gene Symbol FZD1
Uniprot