Recombinant Human EphA5 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human EphA5 Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human EphA5 at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin-A2 Fc Chimera with a linear range of 0.16-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human EphA5 protein
Ser56-Pro573, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser56
Protein/Peptide Type
Recombinant Proteins
Gene
EPHA5
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
58.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-85 kDa, reducing conditions
Publications
Read Publications using
3036-A5 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EphA5 Protein, CF

  • Bsk
  • Cek7
  • EC 2.7.10
  • EC 2.7.10.1
  • Ehk1
  • EHK-1
  • EHK1Hek7
  • EK7
  • EPH homology kinase 1
  • Eph homology kinase-1
  • EPH receptor A5
  • EphA5
  • EPH-like kinase 7
  • ephrin type-A receptor 5
  • Hek7
  • receptor protein-tyrosine kinase HEK7
  • Rek7
  • TYRO4HEK7CEK7
  • tyrosine-protein kinase receptor EHK-1

Background

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7, is a member of the Eph receptor tyrosine kinase family which binds members of the Ephrin ligand family (1‑4). The A and B class Eph proteins have a common structural organization. Activation of kinase activity occurs after membrane-bound or clustered ligand recognition and binding. Reverse signaling is propagated through the Ephrin ligand. The human EphA5 cDNA encodes a 1037 amino acid (aa) precursor including a 24 aa signal sequence, a 549 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 443 aa cytoplasmic domain (5). The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (5). Multiple splice variants of human EphA5 are produced in brain and differ in their extracellular and cytoplasmic sequences (6). Taking into account differences in splice variants between species, comparable regions of the human EphA5 ECD share greater than 96% aa sequence identity with bovine, mouse, and rat EphA5, approximately 40% aa identity with human EphA1 and EphA2, and 54‑61% aa identity with human EphA3, 4, 7, and 8. EphA5 is widely expressed in neurons of the adult human CNS (6), with the exception of few areas such as the substantia nigra (7). In rat, the expression of EphA5 during embryogenesis suggests a role in the development of CNS neurons (8).
  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev 15:419.
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Fox, G.M. et al. (1995) Oncogene 10:897.
  6. Miescher, G.C. et al. (1997) Mol. Brain Res. 46:17.
  7. Olivieri, G. and G.C. Miescher (1999) J. Histochem. Cytochem. 47:855.
  8. Taylor, V. et al. (1994) Neuroscience 63:163.

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Publications for EphA5 (3036-A5)(4)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, Control, ELISA Capture, Immunization.


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Bioassay
(1)
Control
(1)
ELISA Capture
(1)
Immunization
(1)
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Human
(2)
Mouse
(1)
N/A
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Showing Publications 1 - 4 of 4.
Publications using 3036-A5 Applications Species
JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022;0(0):101817. 2022 [PMID: 35278434] (ELISA Capture, N/A) ELISA Capture N/A
S D'Angelo, FI Staquicini, F Ferrara, DI Staquicini, G Sharma, CA Tarleton, H Nguyen, LA Naranjo, RL Sidman, W Arap, AR Bradbury, R Pasqualini Selection of phage-displayed accessible recombinant targeted antibodies (SPARTA): methodology and applications JCI Insight, 2018;3(9):. 2018 [PMID: 29720567] (Bioassay, Human) Bioassay Human
Staquicini F, Qian M, Salameh A, Dobroff A, Edwards J, Cimino D, Moeller B, Kelly P, Nunez M, Tang X, Liu D, Lee J, Hong W, Ferrara F, Bradbury A, Lobb R, Edelman M, Sidman R, Wistuba I, Arap W, Pasqualini R Receptor tyrosine kinase EphA5 is a functional molecular target in human lung cancer. J Biol Chem, 2015;290(12):7345-59. 2015 [PMID: 25623065] (Immunization, Mouse) Immunization Mouse
Hahn AS, Kaufmann JK, Wies E, Naschberger E, Panteleev-Ivlev J, Schmidt K, Holzer A, Schmidt M, Chen J, Konig S, Ensser A, Myoung J, Brockmeyer NH, Sturzl M, Fleckenstein B, Neipel F The ephrin receptor tyrosine kinase A2 is a cellular receptor for Kaposi&#039;s sarcoma-associated herpesvirus. Nat. Med., 2012;18(6):961-6. 2012 [PMID: 22635007] (Control, Human) Control Human

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Bioinformatics

Gene Symbol EPHA5
Uniprot