Recombinant Human DGCR2 His-tag Protein, CF

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2 μg/lane of Recombinant Human DGCR2 His-tag (Catalog # 10161-DG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-60 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human DGCR2 His-tag Protein, CF Summary

Details of Functionality
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Human DGCR2, immobilized at 2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth. 
Source
Chinese Hamster Ovary cell line, CHO-derived human DGCR2 protein
Glu22-Ala349, with an C-terminal 6-His tag
Accession #
N-terminal Sequence
Glu22
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-60 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human DGCR2 His-tag Protein, CF

  • DGCR2
  • DGS-C
  • DiGeorge syndrome critical region gene 2
  • IDDDKFZp686I1730
  • integral membrane protein deleted in DiGeorge syndrome
  • integral membrane protein DGCR2/IDD
  • KIAA0163
  • KIAA0163DiGeorge syndrome critical region protein 2
  • LAN
  • SEZ-12

Background

DiGeorge Syndrome Critical Region 2 (DGCR2), also known as IDD, DGS-C, LAN, and SEZ-12, is an adhesion receptor protein located on the long arm of chromosome 22 (1). The DGCR2 gene encodes an integral membrane protein, consisting of an extracellular domain, a single transmembrane region and a cytoplasmic tail (1). The mature extracellular domain (ECD) of DGCR2 contains both a C-type lectin domain and a cysteine-rich region similar to that of the low density lipoprotein receptor (LDLR) (1, 3). The mature ECD of human DGCR2 shares 93% and 92% amino acid sequence identity with mouse and rat, respectively. DGCR2 is expressed during neurodevelopment in human brain tissues (3). Deletion of the 22q11.2 region results in an extremely variable disorder called 22q11.2 deletion syndrome, with a phenotype ranging from very mild symptoms to severe intellectual disability, facial dysmorphism, heart defects, and urogenital abnormalities (4). Recent studies suggest that DGCR2 regulates critical steps of early cortico-genesis possibly through a Reelin-dependent mechanism. Deletion of DGCR2 has a pathogenic impact on cortical formation by reducing protein expression level, and it plays a critical role in vulnerability to schizophrenia (5). Furthermore, expression of DGCR2 together with USP18 gene may serve as a progonostic marker for muscle invasive bladder cancer survival in patients (5).
  1. Augusseau, S. et al. (1986) Hum. Genet. 74:206.
  2. Mugikura, S. et al. (2016) Biochem Biophys Rep. 5:120.
  3. Kajiwara, K. et al. (1996) Biochem. Biophyis. Res. Commun. 222:144.
  4. Vaisvilas, M. et al. (2018) Balkan J Med Genet. 21:87.
  5. Molinard-Chenu A. and Dayer A. (2017) Biol. Psych. 83:692.

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