Recombinant Human Desmocollin-2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Desmocollin-2 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells. The ED₅₀ for this effect is 0.150-1.50 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Desmocollin-2 protein
  Arg136-Arg684, with a C-terminal 6-His tag
• Accession #: Q02487
• N-terminal Sequence: Arg136
• Protein/Peptide Type: Recombinant Proteins
• Gene: DSC2
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 62.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Desmocollin-2 Protein, CF

• ARVD11
• Cadherin family member 2
• CDHF2
• CDHF2DGII/III
• desmocollin 2
• Desmocollin2
• Desmocollin-2
• Desmocollin-3
• Desmosomal glycoprotein II
• desmosomal glycoprotein II/III
• Desmosomal glycoprotein III
• DG2
• DSC2
• DSC3DKFZp686I11137

Background

Desmocollin-2 (DSC2) is a 100 kDa - 110 kDa transmembrane glycoprotein member of the cadherin family of calcium dependent adhesion molecules (1 - 3). Human DSC2 is synthesized as a 901 amino acid (aa) precursor. It contains a 108 aa propeptide plus a mature region that consists of a 559 aa extracellular domain (ECD) that contains five cadherin-like domains, a 21 aa transmembrane segment, and a 186 aa cytoplasmic region (4). Within the ECD, human DSC2 shares 74% - 79% aa sequence identity with bovine, mouse, and rat DSC2. It shares 54% and 64% aa sequence identity with Desmocollin-1 and -3, respectively. DSC2 is also expressed as long and short splice forms which differ in their cytoplasmic regions (4, 5). The N-terminal two cadherin-like domains mediate homophilic interactions as well as heterophilic interactions with Desmoglein-2 (6). DSC2 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (1, 5, 7). In contrast to DSC1 and DSC3, DSC2 is also expressed in simple epithelia lining the gastrointestinal tract, liver, and kidney (5). During colon carcinogenesis however, DSC2 is downregulated, while DSC1 and DSC3 are upregulated (8). DSC2 is additionally expressed in the myocardium, and a variety of DSC2 mutations are associated with the cardiac arrhythmia disorder ARVC (5, 9).

1. Kottke, M.D. et al. (2006) J. Cell Sci. 119:797.
2. Garrod, D.R. et al. (2002) Mol. Membrane Biol. 19:81.
3. Leckband, D. and A. Prakasam (2006) Annu. Rev. Biomed. Eng. 8:259.
4. Parker, A.E. et al. (1991) J. Biol. Chem. 266:10438.
5. Nuber, U.A. et al. (1995) Eur. J. Cell Biol. 66:69.
6. Syed, S. et al. (2002) Biochem. J. 362:317.
7. Theis, D.G. et al. (1993) Int. J. Dev. Biol. 37:101.
8. Khan, K. et al. (2006) Br. J. Cancer 95:1367.
9. van Tintelen, J.P. et al. (2007) Curr. Opin. Cardiol. 22:185.

Bioinformatics

• Gene Symbol: DSC2
• Uniprot:
  • Q02487(Human)