Recombinant Human Collagen XXIII alpha 1/COL23A1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Collagen XXIII alpha 1/COL23A1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of SW1353 human chondrosarcoma cells. When 5 x 10⁴ cells/well are added to recombinant human COL23A1 coated plates (10 μg/mL, 100 μL/well), ≥60% will adhere after 60 minutes at 37 °C.
• Source: Mouse myeloma cell line, NS0-derived human Collagen XXIII alpha 1 protein
  Glu111-Lys540, with an N-terminal 6-His tag
• Accession #: NP_775736
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: COL23A1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 41.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-65 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Collagen XXIII alpha 1/COL23A1 Protein, CF

• COL23A1
• collagen alpha-1(XXIII) chain
• Collagen XXIII alpha 1
• collagen, type XXIII, alpha 1
• DKFZp434K0621
• procollagen, type XXIII, alpha 1

Background

Collagen XXIII alpha 1 (sometimes abbreviated COL23A1) is a ~75 kDa type II transmembrane nonfibrillar collagen that is a member of the collagenous transmembrane protein superfamily (1, 2). This family also includes collagens XIII, XVII, XXV and non-collagens with triple-helical regions such as ectodysplasin A, class A macrophage scavenger receptors, and MARCO (2). The human Collagen XXIII mRNA encodes a 540 amino acid (aa) protein containing a 34 aa N-terminal cytoplasmic domain, a 21 aa transmembrane (TM) domain and a 485 aa extracellular domain (ECD). The ECD contains a coiled-coil consensus sequence to aid homotrimerization (aa 64 - 69), a furin cleavage site (aa 105 - 110), a pair of cysteines thought to form intermolecular disulfides (aa 106 and 108), and three collagen domains (1, 3 - 5). The C-terminal 20 aa, including cysteines at aa 525 and 537 of Collagen XXIII, is conserved among TM collagen proteins. Proteolytic cleavage, occurring mainly in the golgi, allows the Collagen XXIII ectodomain to be secreted as a soluble trimer of ~60 kDa subunits (1, 5). Cell surface cleavage can also occur but is slow, presumably due to presence of Collagen XXIII in lipid raft membrane domains (5). The protein database includes three variants of 537, 316 and 309 aa with various portions missing or substituted; all appear to lack TM segments (6). The human Collagen XXIII ECD shares 92%, 93%, 85%, 85% and 84% aa identity with mouse, rat, canine, equine and bovine Collagen XXIII, respectively. Collagen XXIII is concentrated at sites of cell contact in epithelia, and is thought to be an adhesion molecule (2, 4). Its upregulation has been correlated with aggressiveness in transformed cells, particularly in prostate cancer (1, 7).

1. Banyard, J. et al. (2003) J. Biol. Chem. 278:20989.
2. Franzke, C-W. et al. (2005) J. Biol. Chem. 280:4005.
3. Snellman, A. et al. (2007) J. Biol. Chem. 282:14898.
4. Koch, M. et al. (2006) J. Biol. Chem. 281:21546.
5. Veit, G. et al. (2007) J. Biol. Chem. 282:27424.
6. Entrez Accession # EAW53833, EAW53834, and AAH42428.
7. Banyard, J. et al. (2007) Clin. Cancer Res. 13:2634.

Bioinformatics

• Gene Symbol: COL23A1
• Uniprot:
  • NP_775736()