Recombinant Human CLC Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human CLC Protein Summary

• Details of Functionality: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells transfected with human CNTF R alpha . The ED₅₀ for this effect is 3‑15 ng/mL.
• Source: E. coli-derived human CLC protein
  Leu28-Phe225, with an N-terminal Met
• Accession #: Q9UBD9
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: CLCF1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 22.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CLC Protein

• B-cell stimulating factor 3
• B-cell stimulatory factor 3
• BSF-3
• BSF3BSF-3
• cardiotrophin-like cytokine factor 1
• CISS2
• CLC
• CLCF1
• CLCNNT-1
• CRLF1 associated cytokine-like factor 1
• neurotrophin-1/B-cell stimulating factor-3
• NNT-1
• NNT1B-cell-stimulating factor 3
• Novel neurotrophin-1
• NR6

Background

Cardiotrophin-like cytokine (CLC), also referred to as novel neurotrophin-1 (NNT-1) or B cell-stimulating factor-3 (BSF-3), is a new member of the IL-6 family of structurally related cytokines that includes IL-6, CNTF, LIF, CT-1, IL-11 and OSM. All family members share the receptor subunit gp130 that belong to the type I cytokine receptor superfamily. Ligand binding leads to gp130 homodimerization or heterodimerization (with LIF receptor or OSM receptor beta ), and induces cell signaling and functional activity. For several family members, including CNTF, IL-6, and IL-11, binding of the ligand to a specific receptor alpha subunit (CNTF R alpha , IL-6 R alpha , or IL-11 R alpha ) is required prior to gp130 homo- or hetero-dimerization.

CLC cDNA encodes a 225 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is cleaved to yield the mature protein. Among the IL-6 family, CLC is the most homologous to cardiotrophin, having 29% aa sequence homology. CLC has been shown to bind with the soluble orphan receptor cytokine-like factor-1 (CLF) to form a heterodimeric composite cytokine that subsequently interacts with the membrane‑associated CNTF R alpha to initiate gp130-LIF R dimerization and cell signaling. Alternatively, when co-expressed within the cell, CLC can complex with soluble CNTF R alpha to form an alternate composite cytokine that is secreted. This composite cytokine is also capable of initiating gp130-LIF R dimerization. The E. coli-expressed CLC can initiate cell signaling via the tripartite membrane‑associated CNTF R alpha and gp130-LIF R. E. coli-expressed CLC does not bind efficiently with soluble CNTF R alpha in solution to initiate cell signaling on cells expressing gp130 and LIF R.

1. Shi, Y. et al. (1999) Biochem. Biophys. Res. Commun. 262:132.
2. Senaldi, G. et al. (1999) Proc. Natl. Acad. Sci. USA 96:11458.
3. Lelievre, E. et al. (2001) J. Biol. Chem. 276:22476.
4. Plun-Favreau, H. et al. (2001) EMBO J. 20:1692.

Publications for CLC (962-CL)(2)

Publications using 962-CL

• M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells International Journal of Molecular Sciences, 2022-01-27;23(3):. 2022-01-27 [PMID: 35163371] (Bioassay, Human)
• Z Wang, M Cui, AM Shah, W Tan, N Liu, R Bassel-Dub, EN Olson Cell-Type-Specific Gene Regulatory Networks Underlying Murine Neonatal Heart Regeneration at Single-Cell Resolution Cell Reports, 2020-12-08;33(10):108472. 2020-12-08 [PMID: 33296652] (Cell Culture, Human)

Bioinformatics

• Gene Symbol: CLCF1
• Uniprot:
  • Q9UBD9()