Recombinant Human CHL-1/L1CAM-2 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CHL-1/L1CAM-2 Protein, CF Summary

• Details of Functionality: Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 100 ng on a nitrocellulose-coated microplate.
• Source: Mouse myeloma cell line, NS0-derived human CHL-1/L1CAM-2 protein Ile25-Gln1096, with a C-terminal 10-His tag
• Accession #: EAW63869
• N-terminal Sequence: Ile25
• Protein/Peptide Type: Recombinant Proteins
• Gene: CHL1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 121.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 160-180 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CHL-1/L1CAM-2 Protein, CF

• CALL
• CALLClose homolog of L1
• cell adhesion molecule with homology to L1CAM (close homolog of L1)
• cell adhesion molecule with homology to L1CAM (close homologue of L1)
• CHL1
• CHL-1
• FLJ44930
• L1CAM-2
• L1CAM2L1 cell adhesion molecule 2
• MGC132578
• neural cell adhesion molecule L1-like protein

Background

Close homolog of L1 (CHL-1), also known as cell adhesion L1-like (CALL) and L1 cell adhesion molecule 2 (L1-CAM2), belongs to the L1 subfamily of the Ig superfamily cell adhesion molecules, which also include L1, neurofascin and NgCAM-related cell adhesion molecule (NrCAM) (1 - 3). These molecules are type I transmembrane proteins that have 6 Ig-like domains and 4 - 5 fibronectin type III-like (FNIII) domains in their extracellular regions. They also shared a highly conserved cytoplasmic region of approximately 110 amino acid residues (aa) containing an ankyrin-binding site. CHL-1 is expressed as a highly glycosylated 185 kDa transmembrane protein by subpopulations of neurons and glia of the central and peripheral nervous system (4, 5). Ectodomain shedding via the metalloprotease-disintegrin ADAM8 releases 165 kDa and 125 kDa soluble CHL-1 fragments, which can diffuse away to function at distant sites (6). CHL-1 is not capable of homotypic interactions, but an extracellular binding partner of CHL-1 has not been identified (4). Human CHL1 has been mapped to chromosome 3p26 and is a candidate gene for 3p - syndrome characterized by mental impairment (7). A missense CHL1 polymorphism associated with an increased risk of schizophrenia has been reported (8). The functional importance of CHL-1 in the nervous system is also evident in CHL-1 deficient mice, which display behavioral abnormalities and show misguided axons within the hippocampus and olfactory tract (9). Enhanced ectodomain-shedding of CHL-1 is also observed in Wobbler mice, the neurodegenerative mutant mice (6). In vitro, soluble or substrate-coated CHL-1 promotes neurite outgrowth and neuronal survival of both cerebellar and hippocampal neurons. Cell surface CHL-1 interacts with integrins in cis to potentiate integrin-dependent cell migration toward extracellular matrix proteins (10). For this enhanced cell motility, CHL-1 linkage to the actin cytoskeleton via interaction between ankyrin and the CHL-1 cytoplasmic region is required.

1. Moos, M. et al. (1988) Nature 334:701. 
2. Holm, J. et al. (1996) Eur. J. Neusci. 8:1613. 
3. Wei, M. et al. (1998) Hum. Genet. 103:355. 
4. Hillenbrand, R. et al. (1999) Eur. J. Neurosci. 11:813. 
5. Liu, Q. et al. (2000) J. Neurosci. 20:7682. 
6. Naus, S. et al. (2004) J. Biol. Chem. 279:16083.
7. Angeloni, D. et al. (1999) Am. J. Med. Genet. 86:482.
8. Sakurai, K. et al. (2002) Mol. Psychiatry 7:412.
9. Montag-Sallaz, M. et al. (2002) Mol. Cell. Biol. 22(22):7967.
10. Buhusi, M. et al. (2003) J. Biol. Chem. 278(27):25024.

Publications for CHL-1/L1CAM-2 (2126-CH)(1)

Publication using 2126-CH

• KF Karstens, E Bellon, A Polonski, G Wolters-Ei, N Melling, M Reeh, JR Izbicki, M Tachezy Expression and serum levels of the neural cell adhesion molecule L1-like protein (CHL1) in gastrointestinal stroma tumors (GIST) and its prognostic power Oncotarget, 2020-03-31;11(13):1131-1140. 2020-03-31 [PMID: 32284790] (ELISA Standard, Human)

Bioinformatics

• Gene Symbol: CHL1
• Uniprot:
  • EAW63869()