Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein, CF

When 0.5 µg/mL (100 µL/well) of Biotinylated Recombinant Human CEACAM‑1/CD66a His-tag Avi-tag Protein (Catalog # AVI2244) is immobilized onto a Streptavidin coated plate (CP004), Recombinant Human CEACAM‑5/CD66e Fc Chimera (10449-CM) binds with an ED50 of 50.0-500 ng/mL

2 μg/lane of Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein (Catalog # AVI2244) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When 0.5 µg/mL (100 µL/well) of Biotinylated Recombinant Human CEACAM-1/CD66a His-tag Avi-tag (Catalog # AVI2244) is immobilized onto a Streptavidin coated plate (Catalog # CP004), Recombinant Human CEACAM-5/CD66e Fc Chimera (Catalog # 10449-CM) binds with an ED₅₀ of 50.0-500 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human CEACAM-1/CD66a protein
  

  Human CEACAM-1 (Gln35-Gly428) Accession # P13688.2	6-His tag	Avi-tag
  N-terminus		C-terminus

• Accession #: P13688.2
• N-terminal Sequence: Gln35
• Structure / Form: Biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 85-105 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein, CF

• antigen CD66
• BGP1
• BGP-1
• BGP1BGPBiliary glycoprotein 1
• BGPI
• Biliary Glycoprotein 1
• biliary glycoprotein adhesion molecule
• carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein)
• carcinoembryonic antigen-related cell adhesion molecule 1
• CD66a antigen
• CD66a
• Cea-1
• CEACAM1
• CEACAM-1
• Hv-1
• Hv-2
• MHVR

Background

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1), also known as cluster of differentiation 66a (CD66a) and biliary glycoprotein (BGP), is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. The CEACAM family is involved in diverse cellular functions from cell adhesion and differentiation to proliferation, and survival and are utilized by several bacterial pathogens to bind and invade host cells (1-3). Mature human CEACAM-1 consists of an extracellular domain (ECD) with 1 V-type Ig-like domain and 3 C2-type Ig-like domains, a transmembrane domain, and a cytoplasmic region shows one ITIM motif and a calmodulin binding site (4). CEACAM-1 is unique among the CEACAM family as it is the only family member to contain the inhibitory ITIM domains. The ECD of human CEACAM-1 shares 54% and 52% amino acid sequence identity with mouse and rat CEACAM-1, respectively. Several alternative splice variants of CEACAM-1 have been reported with alterations occurring in both the ECD and cytoplasmic region, though the function of the isoforms remain poorly understood (5). CEACAM-1 can be expressed in human epithelial, endothelial, and hematopoietic cells and is involved in morphogenesis, apoptosis, angiogenesis, and cell proliferation as well as playing a role in innate and adaptive immunity (6,7). CEACAM-1 functions through either homophilic interactions with CEACAM-1 variants or binds in a heterophilic manner to other CEACAMs, including CEACAM-5, CEACAM-6, and CEACAM-8 (7). Several pathogens from Escherichia coli and Neisseria gonorrhoeae to N. meningitidis use CEACAM-1 as a ligand as a step in bacterial invasion of the host (8). CEACAM-1 signaling is associated with inhibition of proliferation and misregulation of its expression is often reported in cancer (5, 9, 10). As such, CEACAM-1 is being studied as a clinical biomarker and/or promising therapeutic target several cancer types including: non-small cell lung cancer, pancreatic, colorectal, bladder, and melanomas (5, 9, 10). Our Avi-tag Biotinylated Recombinant Human CEACAM‑1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Tchoupa, A. et al. (2014) J. Cell Commun. Signal 12:27.
2. Hauck, C.R. et al. (2006) Eur. J. Cell Biol. 85:235
3. Kuespert, K. et al. (2006) Curr Opin. Cell Biol. 18:565.
4. Kim, W.M. et al. (2019) Seminars in immunology 42:101296.
5. Dankner, M. et al. (2017) Oncoimmunology. 6:e1328336.
6. Gandhi, A.K. et al. (2021) Commun Biol 4:360.
7. Helfrich, I. and Singer, B.B. (2019) Cancers 11:356.
8. Voges, M. et al. (2010) BMC Microbiol. 10:117.
9. Beauchemin, N. and Arabzadeh, A. (2013) Cancer Metastasis Rev. 32:643.
10. Fiori, V. et al. (2012) Ann Ist Super Sanita. 48:161.

Bioinformatics

• Uniprot:
  • P13688.2()