Recombinant Human CD84/SLAMF5 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human CD84/SLAMF5 Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using PHA stimulated human T cells in the presence of anti-CD3. Tangye, S.G. et al. (2003) J. Immunol. 171:2485. The ED50 for this effect is 1-4 μg/mL in the presence of anti-CD3 immobilized at least at 20 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human CD84/SLAMF5 protein
Lys22-Arg220, with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Lys22
Protein/Peptide Type
Recombinant Proteins
Gene
CD84
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
23.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-43 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD84/SLAMF5 Protein, CF

  • CD84 antigen (leukocyte antigen)
  • CD84 antigen
  • CD84 molecule
  • CD84
  • Cell surface antigen MAX.3
  • DKFZp781E2378
  • hCD84
  • hly9-beta
  • leucocyte differentiation antigen CD84
  • leukocyte antigen CD84
  • Leukocyte differentiation antigen CD84
  • Ly-9B
  • mCD84
  • Signaling lymphocytic activation molecule 5
  • SLAM family member 5
  • SLAMF5
  • SLAMF5LY9B

Background

CD84, also known as Ly-9B and SLAMF5, is a type I transmembrane protein in the SLAM subgroup of the CD2 family. SLAM family proteins regulate multiple aspects of immune system function (1). Mature human CD84 consists of a 204 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 99 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs (ITSMs) (2, 3). Alternate splicing generates a soluble ECD, an isoform that lacks the first Ig-like domain, and additional isoforms with deletions in the cytoplasmic domain (4). CD84 exhibits homophilic binding which is mediated by the N-terminal Ig-like domain (5). Ligation induces tyrosine phosphorylation in the cytoplasmic ITSMs which then recruit the signaling adaptor molecules SAP (SLAM‑associated protein) and EAT-2 (EWS/Fli1-activated transcript 2) (6, 7). CD84 is expressed as a 60-90 kDa molecule with extensive and cell type-specific glycosylation (2, 3, 8). It is widely expressed among hematopoietic cells including hematopoietic stem cells (8), myeloid cells (e.g. macrophages, monocytes, dendritic cells, granulocytes, and mast cells) (3, 6, 8‑10), platelets and megakaryocytes (3, 5, 8, 11), and lymphocytes. Within the T cell lineage, CD84 is expressed on thymocytes, CD4-CD8- cells, single positive CD4 or CD8 cells, NKT cells, and on mouse but not human NK cells (5, 6, 8, 9, 12). Within the B cell lineage, it is expressed on pro- and pre-, mature, marginal zone, and memory B cells as well as plasma cells (6, 8, 13). CD84 signaling inhibits Fc epsilon RI-induced mast cell activation (10) but enhances platelet activation (11), LPS-induced macrophage activation (8), T cell proliferation and IFN-gamma production (5, 7), and the interactions between T cells and B cells that are required for germinal center formation (14).
  1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol.  29:665.
  2. de la Fuente, M.A. et al. (1997) Blood. 90:2398.
  3. Krause, S.W. et al. (2000) Biochem. J. 346:729.
  4. Palou, E. et al. (2000) Tissue Antigens 55:118.
  5. Martin, M. et al. (2000) J. Immunol. 167:3668.
  6. Tangye, S.G. et al. (2002) Eur. J. Immunol. 32:1640.
  7. Tangye, S.G. et al. (2003) J. Immunol. 171:2485.
  8. Sintes, J. et al. (2010) J. Leukoc. Biol. 88:687.
  9. Romero, X. et al. (2004) Tissue Antigens 64:132.
  10. Alvarez-Errico, D. et al. (2011) J. Immunol. 187:5577.
  11. Nanda, N. et al. (2005) Blood 106:3028.
  12. Wang, N. et al. (2010) J. Immunol. 185:5683.
  13. De Salort, J. et al. (2011) Immunol. Lett. 134:129.
  14. Cannons, J.L. et al. (2010) Immunity 32:253.

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Bioinformatics

Gene Symbol CD84
Uniprot