Recombinant Human CD5L Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human CD5L Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit apoptosis on the J774A.1 mouse reticulum cell sarcoma macrophage cells. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413. The presence of rhCD5L inhibited rhFas Ligand induced apoptosis by 70-90%. The ED50 for this effect is 1-4 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human CD5L protein
Ser20-Gly347, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ser20
Protein/Peptide Type
Recombinant Proteins
Gene
CD5L
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
43 kDa, reducing conditions
Publications
Read Publications using
2797-CL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human CD5L Protein, CF

  • AIM
  • API6
  • apoptosis inhibitor 6
  • CD5 antigen-like (scavenger receptor cysteine rich family)
  • CD5 antigen-like
  • CD5 molecule-like
  • CD5L
  • CT-2
  • IgM-associated peptide
  • PRO229
  • Spalpha
  • SP-alpha

Background

CD5L (CD5 antigen-like), also known as Sp alpha and AIM, is a 50 kDa secreted glycoprotein that belongs to the SRCR (scavenger receptor cysteine rich) group B family of proteins. Group B proteins are distinguished by SRCR domains that are encoded by a single exon (1 - 3). The human CD5L cDNA encodes a 347 amino acid (aa) precursor that includes a 19 aa signal sequence and three SRCR domains (4, 5). Among group B proteins, CD5L is most closely related to CD5 and CD6, with which it shares 18% and 31% aa sequence identity, respectively. CD5L is up-regulated in macrophages at inflammatory sites. It sustains inflammatory reactions by both increasing the phagocytic capacity of macrophages and impeding the apoptosis of local macrophages, NK cells, and T cells (6, 7). Agonists of the LXR and RXR nuclear hormone receptors induce CD5L upregulation in macrophages and reduce macrophage apoptosis (8, 9). Oxidized LDL (which acts through LXR/RXR) is taken up by macrophages, promoting their development into foam cells. The increased level of CD5L protects foam cells from apoptosis but permits more rapid cellular accumulation and atherosclerotic plaque formation (9). In activated B cells, however, the combination of CD5L and TGF-beta inhibits proliferation. The binding of CD5L to splenic B cells is increased following TGF-beta exposure, suggesting that TGF-beta increases the expression or availability of an unidentified CD5L receptor (5, 10). CD5L also functions as a pattern recognition molecule by binding both lipoteichoic acid on Gram positive and lipopolysaccharide on Gram negative bacteria (11). In the thymic cortex, CD5L protects cortical CD4+CD8+ thymocytes from apoptosis (12). CD5L circulates in the serum in complex with IgM (13).

  1. Sarrias, M.R. et al. (2004) Crit. Rev. Immunol. 24:1.
  2. Resnick, D. et al. (1994) Trends Biochem. Sci. 19:5.
  3. Mukhopadhyay, S. and S. Gordon (2004) Immunobiology 209:39.
  4. Gebe, J.A. et al. (1997) J. Biol. Chem. 272:6151.
  5. Gebe, J.A. et al. (2000) Immunol. 99:78.
  6. Haruta, I. et al. (2001) J. Biol. Chem. 276:22910.
  7. Kuwata, K. et al. (2003) Am. J. Pathol. 162:837.
  8. Valledor, A.F. et al. (2004) Proc. Natl. Acad. Sci. 101:17813.
  9. Arai, S. et al. (2005) Cell Metab. 1:201.
  10. Yusa, S. et al. (1999) Eur. J. Immunol. 29:1086.
  11. Sarrias, M-R. et al. (2005) J. Biol. Chem. 280:35391.
  12. Miyazaki, T. et al. (1999) J. Exp. Med. 189:413.
  13. Tissot, J.D. et al. (2002) Electrophoresis 23:1203.

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2797-CL
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Publications for CD5L (2797-CL)(2)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 2 applications: Bioassay, ELISA Standard.


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Bioassay
(1)
ELISA Standard
(1)
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N/A
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Bioinformatics

Gene Symbol CD5L
Entrez
Uniprot