Recombinant Human Casein Kinase 2 alpha Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Casein Kinase 2 alpha Protein, CF Summary

• Source: E. coli-derived human Casein Kinase 2 alpha protein
  Asp253-Gln391, with an N-terminal Met and C-terminal 6-His tag
• Accession #: P68400-1
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Enzymes
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Theoretical MW: 16 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 14-16 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a 0.2 μm filtered solution in MES, NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Casein Kinase 2 alpha Protein, CF

• Casein Kinase 2 alpha
• casein kinase 2, alpha 1 polypeptide
• casein kinase II alpha 1 subunit
• CK II alpha
• CK2 catalytic subunit alpha
• CK2A1
• CK2A1casein kinase II subunit alpha
• CKII
• CSNK2A1
• EC 2.7.11
• EC 2.7.11.1
• protein kinase CK2

Background

Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein (1). It is involved in various cellular processes, including cell cycle control, apoptosis, and circadian rhythm (2). The kinase exists as a tetramer and is composed of an alpha , an alpha ', and two beta subunits (3). The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The Recombinant Human Casein Kinase 2 alpha contains amino acid 253 to 391 of the alpha subunit, which includes an O-GlcNAcylation site at Ser347 (4). O-GlcNAcylation of Casein Kinase 2 alpha not only regulates its kinase substrate specificity, but also regulates the stability of the protein. The Recombinant Human Casein Kinase 2 alpha is an ideal model protein for studying O-GlcNAcylation. It can be O-GlcNAcylated by OGT, the enzyme that introduces O-GlcNAc to target proteins. The introduced O-GlcNAc residue can be detected by Recombinant Human B3GALNT2 (Cat# 1848-GT), a GalNAc transferase that is highly active on O-GlcNAc (5).

1. Ahmad, K.A. et al. (2008) Adv. Enzyme Regul. 48:179.
2. Rabalski, A.J. et al. (2016) Clinical Cancer Research 22:2840.
3. Litchfield, D.W. (2003) Biochem. J. 369:1.
4. Tarrant, M.K. et al. (2012) Nat. Chem. Biol. 8:262.
5. Wu, Z.L. et al. (2018) Cell Chem. Biol. DOI:https://doi.org/10.1016/j.chembiol.2018.07.007.

Bioinformatics

• Uniprot:
  • P68400-1()