Recombinant Human BTN3A3 His-tag Protein, CF

Recombinant Human BTN3A3 (Catalog # 1350-BT) inhibits anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect is 1-6 μg/mL.

2 μg/lane of Recombinant Human BTN3A3 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 28 -37 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BTN3A3 His-tag Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED₅₀ for this effect is 1-6 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human BTN3A3 protein
  Gln30-Trp248, with a C-terminal 6-His tag
• Accession #: O00478-1
• N-terminal Sequence: Gln30
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-37 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BTN3A3 His-tag Protein, CF

• BTF3
• BTF3butyrophilin 3
• BTN3.3
• BTN3A3
• butyrophilin subfamily 3 member A3
• butyrophilin, subfamily 3, member A3

Background

BTN3A3 (Butyrophylin subfamily 3 member A3), is a type 1 transmembrane glycoprotein belonging to the Ig superfamily. BTN molecules have three subfamilies, BTN1-3 and are composed of two Ig domains, a single transmembrane domain, and a large C-terminal domain located in the cell cytoplasm (1, 2). Mature human BTN3A3 consists of a 219 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 315 aa cytoplasmic domain. Within the ECD, human BTN3A3 shares 45% and 41% aa sequence identity with mouse and rat BTN3A3, respectively. The BTN3A subfamily of butyrophilins (BTN3A1, BTN3A2 and BTN3A3) are expressed by most human immune cell subsets, including T cells, B cells, monocytes, dendritic cells and natural killer (NK) cells (2, 3). In in vitro studies, the BTN3A-specific monoclonal antibody bound to BTN3A directly on the T cell surface, driving the phosphorylation of the subfamily member BTN3A3, and initiating an inhibitory signaling cascade that resulted in decreased T cell proliferation and cytokine production (2). Single nucleotide polymorphisms (SNPs) in BTN3A3 was reported to be associated with increased susceptibility to ovarian cancer, supporting the notion that butyrophilins might contribute to immune evasion in oncology settings (4).

1. Rhodes, D.A. et al. (2015) J. Immunol. 194:2390.
2. Yamashiro, H. et al. (2010) J. Leukoc. Biol. 88:757.
3. Messal, N. et al. (2011) Eur. J. Immunol. 41:3443.
4. Peedicayil, A. et al. (2010) PLoS ONE 5:e8884.

Bioinformatics

• Uniprot:
  • O00478-1()