Recombinant Human BAI3 Protein, CF

When Recombinant Human C1qL3 (Catlog # 9115-TN) is coated at 1 µg/mL (100 μL/well), Recombinant Human BAI3 (Catlog # 9106-BA) binds with a typical ED50 of 0.3-1.8 μg/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human BAI3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human C1qL3 (Catalog # 9115-TN) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human BAI3 that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.
• Source: Human embryonic kidney cell, HEK293-derived human BAI3 protein
  Ala25-Thr880, with C-terminal 6-His tag
• Accession #: NP_001695
• N-terminal Sequence: Ala25
• Protein/Peptide Type: Recombinant Proteins
• Gene: BAI3
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 109-132 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 1 mg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human BAI3 Protein, CF

• BAI3
• brain-specific angiogenesis inhibitor 3
• dJ91B17.1 (brain-specific angiogenesis inhibitor 3)
• KIAA0550MGC133100

Background

Brain angiogenesis inhibitor 3 (BAI3) is an approximately 180 kDa adhesion GPCR that plays a role in neuronal synapse formation and maintenance. Mature human BAI3 consists of an 856 amino acid (aa) N-terminal extracellular domain (ECD) with one CUB domain, four TSP1 domains, and a GPS protease sensitive linker, followed by a region with seven transmembrane segments and a 376 aa C-terminal cytoplasmic domain (1, 2). Within the N-terminal ECD, human BAI3 shares 98% and 97% aa sequence identity with mouse and rat BAI3, respectively. BAI3 is primarily expressed post-synaptically in the cerebral cortex and on cerebellar Purkinje cells (1-5), and it binds to the TNF-like proteins C1qL1, 2, 3, and 4 (6). BAI3 interaction with C1qL1 is required for the formation and maintenance of excitatory synapses between climbing fibers and parallel fibers with Purkinje cells (4, 5). BAI3 ligation can also reduce excitatory synaptic density in hippocampal neurons, decrease dendrite arborization, and promote axon pruning in climbing fibers (3, 4, 6). It is additionally expressed in developing muscle where it is required for myoblast fusion (7).

1. Shiratsuchi, T. et al. (1997) Cytogenet. Cell Genet. 79:103.
2. Kee, H.J. et al. (2004) FEBS Lett. 569:307.
3. Lanoue, V. et al. (2013) Mol. Psychiatry 18:943.
4. Kakegawa, W. et al. (2015) Neuron 85:316.
5. Sigoillot, S.M. et al. (2015) Cell Rep. 10:820.
6. Bolliger, M.F. et al. (2011) Proc. Natl. Acad. Sci. USA 108:2534.
7. Hamoud, N. et al. (2014) Proc. Natl. Acad. Sci. USA 111:3745.

Bioinformatics

• Gene Symbol: BAI3
• Uniprot:
  • NP_001695()