Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

When Recombinant Human Angiopoietin‑like Protein 2/ANGPTL2 (Catalog # 9795-AN) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) binds with an ED50 of 6-48 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF Summary

• Additional Information: C-Terminal Fragment
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Angiopoietin‑like Protein 2/ANGPTL2 is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human LILRB2/CD85d/ILT4 Fc Chimera Recombinant Human ILT4/CD85d Fc Chimera (Catalog # 2078-T4) that produces 50% of the optimal binding response is 6-48 ng/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Angiopoietin-like Protein 2/ANGPTL2 protein
  Thr260-His493, with a C-terminal 6-His tag
• Accession #: Q9UKU9.1
• N-terminal Sequence: Thr260
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-36 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Angiopoietin-like Protein 2/ANGPTL2, CF

• angiopoietin-like 2
• Angiopoietin-like Protein 2
• angiopoietin-related protein 2
• ANGPTL2
• ANGRP2
• ARP2HARP
• MGC8889

Background

Angiopoietin-like Protein 2 (ANGPTL2), also known as ARP2 is a secreted 56 kDa glycoprotein that contains a N-terminal coiled coil domain and a C-terminal fibrinogen like domain (1). ANGPTL2 is one of the seven members of the Angiopoietin Like family proteins, which are structurally similar to angiopoietins (2). Within mature human ANGPTL2, amino acid 260-493 contains the C-terminal fibrinogen like domain, and this domain shares 99% sequence identity with both mouse and rat homologs. ANGPTL2 is widely expressed in many tissues, and its expression is induced by chronic, but not acute hypoxia (3). Elevated level of ANGPTL2 in the serum is also correlated with inflammation and obesity (3). ANGPTL2 has both pro and anti-angiogenic functions (1, 4). It serves as tumor suppressor in ovarian cancer (5), and also promote metastasis in various cancer types, making it potential biomarker for tumor progression (6-7). ANGPTL2 also function as growth factor to enhance the survival of hematopoietic progenitors, mediated by its coiled coil domain (8). Receptor of ANGPTL2 is recently identified as LILRB2, and both the coiled coil domain and fibrinogen like domain are required for optimal binding (9).

1. Kim, I. et al. (1999) J Biol Chem 274:26523.
2. Santulli, G. et al. (2014) Front Endocrinol 5:4.
3. Tabata, M. et al. (2009) Cell Metab 10:178.
4. Kubota, Y. et al. (2005) Proc. Natl. Acad. Sci. U.S.A. 102:13502.
5. Kikuchi, R. et al. (2008) Cancer Res 68:5067.
6. Odagiri, H. et al. (2014) Sci Signal 7:ra7.
7. Endo, M. et al. (2012) Cancer Res 72:1784.
8. Broxmeyer, H.E. et al. (2012) Blood Cells Mol Dis 48:25.
9. Deng, M. et al. (2014) blood 124:924.

Publications for Angiopoietin-like Protein 2/ANGPTL2 (9795-AN)(1)

Publication using 9795-AN

• L Cui, H Wei, ZM Li, XB Dong, PY Wang TGF-&amp;beta1 aggravates degenerative nucleus pulposus cells inflammation and fibrosis through the upregulation of angiopoietin-like protein 2 expression European review for medical and pharmacological sciences, 2020-12-01;24(23):12025-12033. 2020-12-01 [PMID: 33336720] (Cell Culture, Human)

Bioinformatics

• Uniprot:
  • Q9UKU9.1()