Recombinant Canine SCF Protein

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications Bioactivity

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Recombinant Canine SCF Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1-3 ng/mL.
Source
E. coli-derived canine SCF/c-kit Ligand protein
Lys26-Ala190, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
KITLG
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
2278-SC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Canine SCF Protein

  • c-kit Ligand
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SLF
  • steel factor

Background

Canine SCF (stem cell factor; also known as c-kit ligand) is a type I transmembrane (TM) glycoprotein that plays an important role in a number of fetal and adult developmental processes (1-4). It is synthesized as a 274 amino acid (aa) precursor that contains a 25 aa signal sequence, a 190 aa extracellular region, a 23 aa TM segment and a 36 aa cytoplasmic tail (5). Within the extracellular region there are four potential N-linked glycosylation sites, two intrachain disulfide bonds, and four
alpha -helices. Although the predicted molecular weight is 19 kDa, the native molecule is anywhere from 28-40 kDa in size and reflects both N- and O-linked glycosylation (1). Glycosylation is not necessary for bioactivity (6). The transmembrane form of SCF can be cleaved proteolytically, generating a 165 aa soluble form. Circulating SCF exists as both a monomer and nondisulfide-linked homodimer, with monomer predominating (50% to 75%) (6). Both the soluble and TM forms have bioactivity. Their principal targets may be different, however (7). A second, alternate splice short form of SCF has been identified in other species (1). It is membrane bound but lacks the proteolytic cleavage site found in the long form. Thus, it cannot give rise to a soluble molecule. No such isoform has been reported for canine, but it could be assumed to exist. The ratio of long form to short form varies from tissue to tissue (1). Soluble canine SCF shares 88%, 93%, 86%, 83%, 76%, 76%, 86% and 88% aa sequence identity with porcine, feline, bovine, human, mouse, rat, goat and equine SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes (1).

  1. Broudy, V.C. (1997) Blood 90:1345.
  2. Nakagawa, S. and T. Kitoh (2000) Curr. Opin. Hematol. 7:133.
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
  4. Kang, J. and S.D. Der (2004) Curr. Opin. Immunol. 16:180.
  5. Dunham, S.P. and D.E. Onions (1996) DNA Seq. 6:233.
  6. Hsu, Y-R. et al. (1997) J. Biol. Chem. 272:6406.
  7. Kapur, R. et al. (1998) Blood 91:879.

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Publications for SCF/c-kit Ligand (2278-SC)(3)

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Bioinformatics

Gene Symbol KITLG
Entrez
Uniprot