RBM8A Antibody (4C4)

Product Discontinued

RBM8A Antibody (4C4) Summary

• Immunogen: Full length native protein to human Y14.
• Localization: Cytoplasmic and Nuclear
• Specificity: Y14 (4C4)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBM8A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.5-1.0 ug/ml
• Application Notes: WB: Predicted molecular weight: 21 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for RBM8A Antibody (4C4)

• Binder of OVCA1-1
• BOV-1
• BOV-1A
• BOV-1B
• BOV-1C
• MDS014
• RBM8BRBM8
• ribonucleoprotein RBM8
• Ribonucleoprotein RBM8A
• RNA binding motif protein 8A
• RNA binding motif protein 8B
• RNA-binding motif protein 8A
• RNA-binding protein 8A
• RNA-binding protein Y14
• Y14
• ZNRP
• ZRNP1

Background

Pre-mRNA splicing appears to play an important role in regulation of gene expression. It has been shown in a number of studies that during the splicing process, specific proteins are recruited to the mRNA and assemble into a complex of mRNA-protein near the exon-exon junction. This complex is termed mRNA-protein complex (mRNP), or the exon-exon junction complex. Several proteins were found to be present in this complex including Y14, magoh, Aly/REF, RNPS1, Upf3, and DEK. Y14 is a 174 amino acid RNA-binding protein that contains one RNA-binding domain in the middle of the protein. Y14 binds to the spliced mRNAs in the nucleus and is exported with the mRNA to the cytoplasm. Similar to other proteins in the mRNA-protein complex, Y14 can interact with the mRNA export factor TAP and enhance the export of the mRNAs. Y14 binds to different mRNAs approx.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for RBM8A Antibody (NB100-2294) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Emily Miller WB Human 05/01/2014

Summary

• Application: Western Blot
• Sample Tested: HeLa, Mouse brain
• Species: Human
• Lot: 045K4791

Bioinformatics

• Gene Symbol: RBM8A
• Entrez:
  • 9939(Human)