RBBP9 Antibody (OTI4G3) - Azide and BSA Free

Western Blot: RBBP9 Antibody (OTI4G3) - Azide and BSA Free [NBP2-73828] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBBP9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBBP9.

Immunocytochemistry/Immunofluorescence: RBBP9 Antibody (OTI4G3) - Azide and BSA Free [NBP2-73828] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RBBP9.

Immunohistochemistry: RBBP9 Antibody (OTI4G3) - Azide and BSA Free [NBP2-73828] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RBBP9 mouse monoclonal antibody.

Flow Cytometry: RBBP9 Antibody (OTI4G3) - Azide and BSA Free [NBP2-73828] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-RBBP9 antibody, and then analyzed by flow cytometry.

Flow Cytometry: RBBP9 Antibody (OTI4G3) - Azide and BSA Free [NBP2-73828] - Analysis of Hela cells, using anti-RBBP9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: RBBP9 Antibody (OTI4G3) - Azide and BSA Free [NBP2-73828] - Analysis of Jurkat cells, using anti-RBBP9 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI4G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

RBBP9 Antibody (OTI4G3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse RBBP9 Antibody (OTI4G3) - Azide and BSA Free (NBP2-03025) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human RBBP9 (NP_006597) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBBP9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 20.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RBBP9 Antibody (OTI4G3) - Azide and BSA Free

• B5T-overexpressed gene protein
• Bog
• BOGB5T overexpressed gene protein
• EC 3.-
• Protein BOG
• putative hydrolase RBBP9
• RBBP10
• RBBP-10
• RBBP-9
• retinoblastoma binding protein 9
• Retinoblastoma-binding protein 10
• Retinoblastoma-binding protein 9MGC9236
• retinoma-binding protein 9

Background

RBBP9 is encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBBP9