Recombinant Human RBBP4/RbAp48 Protein

SDS-Page: Recombinant Human RBBP4/RbAp48 Protein [NBP3-18203] - Recombinant Human RBBP4/RbAp48 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at approximately 50 kDa.

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Concentration: LYOPH

Recombinant Human RBBP4/RbAp48 Protein Summary

• Description: A recombinant protein corresponding to the amino acids Met1-Ser425 of Human RBBP4/RbAp48.

  Source: Sf 9 (baculovirus)

  (Accession #: NP_005601.1)
  
  Store at -20C to -80C long term. After reconstitution, the product is stable at -20C for 3 months, and 2-8C for up to 1 week.

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Human RBBP4/RbAp48 Protein at 1 ug/ml (100 ul/well) can bind RBBP4/RbAp48 Rabbit pAb with a linear range of 0.976-7.09 ng/ml.
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: RBBP4
• Purity: >92%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU/ug of the protein by LAL method

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Lyophilized from a 0.22 um filtered solution of 50mM Tris, 500mM NaCl, pH 7.4
• Concentration: LYOPH
• Purity: >92%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water

Alternate Names for Recombinant Human RBBP4/RbAp48 Protein

• CAF-1 p48
• CAF-1 subunit C
• CAF-1C
• CAF-I p48
• Chromatin assembly factor 1 subunit C
• Chromatin assembly factor I p48 subunit
• chromatin assembly factor/CAF-1 p48 subunit
• histone-binding protein RBBP4
• MSI1 protein homolog
• Nucleosome-remodeling factor subunit RBAP48
• NURF55
• RBAP48
• RBBP4
• RBBP-4
• retinoblastoma binding protein 4
• Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
• Retinoblastoma-binding protein p48

Background

RbAp48 encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RBBP4