RBBP4/RbAp48 Antibody (C.15200033) - BSA Free

Western Blot: RBBP4/RbAp48 Antibody (C.15200033) [NBP2-62798] - Analysis of nuclear extracts from HeLa cells (20 ug) using the monoclonal antibody against hRbAp46/48.

• Reactivity: Hu
• Applications: WB
• Clone: C.15200033
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

RBBP4/RbAp48 Antibody (C.15200033) - BSA Free Summary

• Description: Monoclonal antibody raised in mouse against the full length human RbAp48 protein (Retinoblastomabinding protein p48) fused to GST. The antibody recognizes both RbAp48 and RbAp46.
• Immunogen: Human RbAp48 protein (retinoblastoma binding protein p48)
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RBBP4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide and 0.05% ProClin 300
• Purity: Protein G purified

Alternate Names for RBBP4/RbAp48 Antibody (C.15200033) - BSA Free

• CAF-1 p48
• CAF-1 subunit C
• CAF-1C
• CAF-I p48
• Chromatin assembly factor 1 subunit C
• Chromatin assembly factor I p48 subunit
• chromatin assembly factor/CAF-1 p48 subunit
• histone-binding protein RBBP4
• MSI1 protein homolog
• Nucleosome-remodeling factor subunit RBAP48
• NURF55
• RBAP48
• RBBP4
• RBBP-4
• retinoblastoma binding protein 4
• Retinoblastoma-binding protein 4CAF-I 48 kDa subunit
• Retinoblastoma-binding protein p48

Background

RbAp48 (UniProtKB/Swiss-Prot entry Q09028) is a component of several complexes which regulate chromatin metabolism. These include (1) the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; (2) the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; (3) the nucleosome remodelling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; (4) the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and (5) the NURF (nucleosome remodeling factor) complex. Therefore it may target chromatin assembly factors as well as chromatin remodeling factors and histone deacetylases to their histone substrates in a nucleosomal DNA dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RBBP4