RASSF2 Antibody [Alexa Fluor® 488]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

RASSF2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human RASSF2
  Lys100-Gly230
  Accession # P50749
• Specificity: Detects endogenous human and mouse RASSF2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

RASSF2 (Ras association domain-containing protein 2; also RASSF2A) is a 34‑36 kDa member of the RASSF family of proteins. It is widely expressed, and serves as a tumor suppressor by interacting with K-Ras in a GTP-dependent manner, and activating caspase-3 and inducing apoptosis. Human RASSF2 is 326 amino acids (aa) in length. It contains a bipartite NLS (aa 151‑167), a Ras-association domain (aa 176‑264) and a SARAH domain (aa 272‑319) that mediates homo- and heterotypic interactions. Multiple isoforms exist. One shows an insertion of 13 aa after Lys213, a second shows a three aa substitution for aa 305‑326, and a third shows a 20 aa substitution for aa 1‑96, accompanied by a three aa substitution for aa 231‑326. RASSF2 is often found down regulated in lung-derived tumor cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.