RASAL2 Antibody

Western Blot: RASAL2 Antibody [NBP1-82579] - Analysis in human cell line U-251 MG.

Immunocytochemistry/Immunofluorescence: RASAL2 Antibody [NBP1-82579] - Staining of human cell line U-2 OS shows localization to plasma membrane & focal adhesion sites. Antibody staining is shown in green.

Product Discontinued

RASAL2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PRQNSTGQAQIRKVDQGGLGARAKAPPSLPHSASLRSTGSMSVVSAALVAEPVQNGSRSRQQSSSSRESPVPKVRAIQRQQTQQVQSPVDSATMSPVERTAAWVLNNGQY
• Predicted Species: Mouse (95%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RASAL2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-P reactivity reported in (PMID: 25384218).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RASAL2 Antibody

• MGC129919
• NGAP
• ras GTPase-activating protein nGAP
• Ras protein activator like 1
• RAS protein activator like 2
• RAS protein activator-like 2

Background

RASAL2 encodes a protein that contains the GAP-related domain (GRD), a characteristic domain of GTPase-activating proteins (GAPs). GAPs function as activators of Ras superfamily of small GTPases. The protein encoded by this gene is able to complement the defective RasGAP function in a yeast system. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RASAL2 Antibody (NBP1-82579)(2)

Publications using NBP1-82579

• Bao Y, Qian C, Liu MY et al. PRKAA/AMPK alpha phosphorylation switches the role of RASAL2 from a suppressor to an activator of autophagy Autophagy 2021-02-24 [PMID: 33563064]
• Feng M, Bao Y, Li Z et al. RASAL2 activates RAC1 to promote triple-negative breast cancer progression. J. Clin. Invest. 2014-12-01 [PMID: 25384218] (IHC-P, Human)

Bioinformatics

• Gene Symbol: RASAL2
• Entrez:
  • 9462(Human)
• Uniprot:
  • Q9UJF2(Human)