RAP74 Antibody

Western Blot: RAP74 Antibody [NBP1-89922] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-459

Immunocytochemistry/Immunofluorescence: RAP74 Antibody [NBP1-89922] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & microtubules.

Immunohistochemistry-Paraffin: RAP74 Antibody [NBP1-89922] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Immunocytochemistry/Immunofluorescence: RAP74 Antibody [NBP1-89922] - Staining of human cell line U-2 OS shows positivity in nucleus and cytoskeleton (microtubules).

Product Discontinued

RAP74 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEK
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: GTF2F2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for RAP74 Antibody

• BTF4
• General transcription factor IIF 74 kDa subunit
• general transcription factor IIF subunit 1
• general transcription factor IIF, polypeptide 1, 74kDa
• TF2F1
• TFIIF
• TFIIF-alpha
• Transcription initiation factor IIF subunit alpha
• transcription initiation factor RAP74

Background

Enables several functions, including RNA polymerase II general transcription initiation factor activity; phosphatase activator activity; and promoter-specific chromatin binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to virus; and transcription initiation from RNA polymerase II promoter. Located in cell junction and nucleoplasm. Part of transcription factor TFIID complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2F2