Recombinant Human Raf-1 Protein

Product Discontinued

Recombinant Human Raf-1 Protein Summary

• Description: Recombinant protein for Human Raf-1
  
  

  Source:Baculovirus (Sf9 insect cells)
  Amino Acid Sequence:(P04049)

• Preparation Method: Determined to be >85% pure by SDS-PAGE
• Source: Sf 9 (baculovirus)
• Protein/Peptide Type: Recombinant Protein
• Gene: RAF1
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Application Notes: Specific activity: The specific activity of RAF1(EE) was determined to be ~6,000 nmol /min/mg in a coupled assay as per activity assay protocol.
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
• Concentration: 0.1 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human Raf-1 Protein

• cRaf
• c-Raf
• c-Raf-1
• CRAFraf proto-oncogene serine/threonine protein kinase
• EC 2.7.11
• EC 2.7.11.1
• NS5
• Proto-oncogene c-RAF
• RAF proto-oncogene serine/threonine-protein kinase
• RAF
• Raf1
• Raf-1
• Raf-1Oncogene RAF1
• v-raf-1 murine leukemia viral oncogene homolog 1
• v-Raf-1

Background

RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly (1). The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: RAF1