Raf-1 [p Ser259] Antibody

Product Discontinued

Raf-1 [p Ser259] Antibody Summary

• Immunogen: Synthetic peptide (Human) derived from a region of human c-Raf that contains serine 259. The sequence is conserved in mouse and rat.
• Modification: p Ser259
• Specificity: A-Raf (90%) was also shown to react in HCT-8 cells, a human cancer cell line expressing high levels of A-Raf.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Application Notes: WB: Use at a concentration of 1:1,000. Predicted molecular weight: 74 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human. Expected to cross-react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Dulbecco's PBS (pH 7.3), 1.0 mg/ml and 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Raf-1 [p Ser259] Antibody

• cRaf
• c-Raf
• c-Raf-1
• CRAFraf proto-oncogene serine/threonine protein kinase
• EC 2.7.11
• EC 2.7.11.1
• NS5
• Proto-oncogene c-RAF
• RAF proto-oncogene serine/threonine-protein kinase
• RAF
• Raf1
• Raf-1
• Raf-1Oncogene RAF1
• v-raf-1 murine leukemia viral oncogene homolog 1
• v-Raf-1

Background

The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Serine 259 is one of the three constitutive phosphorylation sites of c-Raf in resting cells together with serine 43 and serine 621. Serine 259 is phosphorylated by PKA and is the main mediator of PKA-induced inhibition of c-Raf kinase activity. Phosphorylation of serine 259 blocks the membrane localization of c-Raf.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RAF1
• Entrez:
  • 5894(Human)