Raf-1 [p Ser338] Antibody (pS338 (DPR/2/2A/3A))

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
pS338 (DPR/2/2A/3A)
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Raf-1 [p Ser338] Antibody (pS338 (DPR/2/2A/3A)) Summary

Immunogen
Synthetic peptide GQRDS*SYY*WEIEAS coupled to glutaraldehyde
Modification
p Ser338
Specificity
NB100-66305 recognizes activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339.
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
RAF1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:1000
Application Notes
NB100-66305 may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999). For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other caseinbased blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Publications
Read Publications using NB100-66305.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Raf-1 [p Ser338] Antibody (pS338 (DPR/2/2A/3A))

  • cRaf
  • c-Raf
  • c-Raf-1
  • CRAFraf proto-oncogene serine/threonine protein kinase
  • EC 2.7.11
  • EC 2.7.11.1
  • NS5
  • Proto-oncogene c-RAF
  • RAF proto-oncogene serine/threonine-protein kinase
  • RAF
  • Raf1
  • Raf-1
  • Raf-1Oncogene RAF1
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • v-Raf-1

Background

The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Raf-1 Antibody (NB100-66305)(2)

We have publications tested in 1 application: WB.


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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol RAF1
Entrez
Uniprot