Synthetic peptide GQRDS*SYY*WEIEAS coupled to glutaraldehyde
NB100-66305 recognizes activated Raf-1, binding to the phosphorylated serine residue at position 338. The antibody binds to Raf-1 that has been activated by Ras or Src, but not to unactivated Raf-1. The antibody does not bind to Raf peptides phosphorylated at other sites, including Y341 and S339.
Protein G purified
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NB100-66305 may be used for Western blotting detection of activated Raf-1 as described by Mason et al. (1999). For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other caseinbased blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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