Raf-1 Antibody (563002) [Alexa Fluor® 350]

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 563002
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 350

Raf-1 Antibody (563002) [Alexa Fluor® 350] Summary

• Immunogen: E. coli-derived recombinant human Raf-1
  Asn189-Thr353
  Accession # P04049
• Specificity: Detects human, mouse, and rat Raf-1 at 74 kDa in Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Raf-1 Antibody (563002) [Alexa Fluor® 350]

• cRaf
• c-Raf
• c-Raf-1
• CRAFraf proto-oncogene serine/threonine protein kinase
• EC 2.7.11
• EC 2.7.11.1
• NS5
• Proto-oncogene c-RAF
• RAF proto-oncogene serine/threonine-protein kinase
• RAF
• Raf1
• Raf-1
• Raf-1Oncogene RAF1
• v-raf-1 murine leukemia viral oncogene homolog 1
• v-Raf-1

Background

The Raf serine/threonine kinases are effectors of Ras that function as MAP3Ks in the ERK phosphorylation cascade. Mammals express three Raf proteins: A-Raf, B-Raf, and Raf-1, also known as C-Raf. Human Raf-1 contains three distinct regions; an N-terminal RBD (Ras-binding domain) (aa 56 - 131), followed by two rich segments [a cysteine-finger region (aa 138 - 184) (also called CR1/C1) and a second cysteine-rich region (CR2) (aa 253 - 264)] and a C-terminal Ser/Thr kinase catalytic domain (aa 354 - 611). Active Raf-1 phosphorylates and activates the MAPK kinases MEK1 and 2, which in turn phosphorylate and activate the MAP kinases ERK1 and 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.