Rae-1 Antibody [Unconjugated] - Pan Specific

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Rae‑1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Rae‑1 Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1136) at 5 µg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Rae-1 Antibody [Unconjugated] - Pan Specific Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Rae‑1 gamma
Leu29-Ser231
Accession # O08604
Specificity
Detects mouse Rae‑1 gamma as well as mouse Rae-1 alpha , 1 beta , 1δ and 1 epsilon in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
Raet1a
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF1136 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 Antibody [Unconjugated] - Pan Specific

  • Rae1
  • Rae-1

Background

Rae-1 gamma is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha , beta , δ and epsilon . Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑6).

  1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
  2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
  3. Cerwenka, A. et al. (2000) Immunity 12:721.
  4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  5. Diefenbach, A. et al. (2001) Nature 413:165.
  6. NKG2D and its Ligands, www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rae-1 Antibody (AF1136)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, Western Blot.


Filter By Application
IHC
(4)
IHC-P
(3)
Western Blot
(1)
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Human
(1)
Mouse
(7)
All Species
Showing Publications 1 - 8 of 8.
Publications using AF1136 Applications Species
J Spielmann, L Mattheis, JS Jung, H Rau beta e, M Gla beta , I Bähr, D Quandt, J Oswald, H Kielstein Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer Sci Rep, 2020;10(1):20606. 2020 [PMID: 33244094] (IHC, Mouse) IHC Mouse
C Godbersen-, TA Coupet, Z Grada, SC Zhang, CL Sentman Toxicity Induced by a Bispecific T Cell-Redirecting Protein Is Mediated by Both T Cells and Myeloid Cells in Immunocompetent Mice J. Immunol., 2020;204(11):2973-2983. 2020 [PMID: 32295875] (IHC, Mouse) IHC Mouse
T Kawakami, K Ito, Y Matsuda, M Noda, A Sakurada, Y Hoshikawa, Y Okada, K Ogasawara Cytotoxicity of natural killer cells activated through NKG2D contributes to the development of bronchiolitis obliterans in a murine heterotopic tracheal transplant model Am. J. Transplant, 2017;0(0):. 2017 [PMID: 28251796] (IHC-P, Mouse) IHC-P Mouse
X Qian, C Hu, S Han, Z Lin, W Xiao, Y Ding, Y Zhang, L Qian, X Jia, G Zhu, W Gong NK1.1(-) CD4(+) NKG2D(+) T cells suppress DSS-induced colitis in mice through production of TGF-? J. Cell. Mol. Med, 2017;0(0):. 2017 [PMID: 28224733] (IHC, Mouse) IHC Mouse
S Sheppard, J Guedes, A Mroz, AM Zavitsanou, H Kudo, SM Rothery, P Angelopoul, R Goldin, N Guerra The immunoreceptor NKG2D promotes tumour growth in a model of hepatocellular carcinoma Nat Commun, 2017;8(0):13930. 2017 [PMID: 28128200] (IHC, Mouse) IHC Mouse
Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery. J Leukoc Biol, 2015;97(3):583-98. 2015 [PMID: 25583577] (IHC-P, Human) IHC-P Human
Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice. Immunology, 2014;141(3):401-15. 2014 [PMID: 24708417] (Western Blot, Mouse) Western Blot Mouse
Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease. J. Clin. Invest., 2009;119(3):636-49. 2009 [PMID: 19197141] (IHC-P, Mouse) IHC-P Mouse

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Bioinformatics

Gene Symbol Raet1a
Uniprot