Rae-1 Antibody [Unconjugated] - Pan Specific

Rae-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Rae-1 Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons of primary sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Sciatic Nerve Crush Increases RAE1 in Peripheral Nerve Axons(A) Schematic diagram of sciatic nerve crush injury site in relation to lumbar DRGs (L3, L4, and L5).(B) Raet1 mRNA expression in ipsilateral L3–5 DRG 3 and 7 days after surgery by qPCR. Two-way ANOVA: effect of injury, F(1,8) = 180.95, p 0.05; crush ipsi versus contra, t = 3.678, ∗p 0.05; crush ipsi versus contra, t = 3.342, ∗p 0.05; crush ipsi versus contra, t = 3.678, ∗p 0.05; crush ipsi versus contra, t = 3.342, ∗p < 0.05; ipsi sham versus crush, t = 3.043, ∗p < 0.05 (n = 5 mice per surgery group per time point).(F) (Top) Pan-RAE1 western blot of DRG (L3–5) tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral DRG 3 and 7 days after sham or crush surgery. One-way ANOVA: 3 days, F(3,16) = 0.438, p = 0.7288; 7 days, F(3,20) = 0.053, p = 0.983 (n = 5–6 mice per surgery group per time point).(G) Composite images of RAE1 immunolabeling in full-length contralateral and ipsilateral sciatic nerves taken from three individual mice 3 days after tight sciatic nerve ligation. Arrow, ligation site; arrowhead, proximal to ligation.(H) Maximum projection images of RAE1 (green) co-localization with axonal marker beta -tubulin III (magenta) in sciatic nerve 3 days after tight ligation. Scale bars, 50 μm.See also Figures S3 and S4. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30712871), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, IHC, B/N
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rae-1 Antibody [Unconjugated] - Pan Specific Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Rae‑1 gamma
  Leu29-Ser231
  Accession # O08604
• Specificity: Detects mouse Rae‑1 gamma as well as mouse Rae-1 alpha , 1 beta , 1δ and 1 epsilon in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Raet1a
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 Antibody [Unconjugated] - Pan Specific

• dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1
• Rae1
• Rae-1

Background

Rae-1 gamma is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha , beta , δ and epsilon . Amino acid sequence identity within this family ranges from 88‑95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig-like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI‑linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10‑14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑6).

1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.
2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
3. Cerwenka, A. et al. (2000) Immunity 12:721.
4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
5. Diefenbach, A. et al. (2001) Nature 413:165.
6. NKG2D and its Ligands, www.RnDSystems.com.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rae-1 Antibody (AF1136)(12)

Publications using AF1136

• Kurt Brassington, Peter Kanellakis, Anh Cao, Ban-Hock Toh, Karlheinz Peter, Alex Bobik et al. Crosstalk between cytotoxic CD8+ T cells and stressed cardiomyocytes triggers development of interstitial cardiac fibrosis in hypertensive mouse hearts Frontiers in Immunology 2022-11-22 [PMID: 36483558]
• Sophie Curio, Sarah C Edwards, Toshiyasu Suzuki, Jenny McGovern, Chiara Triulzi, Nagisa Yoshida et al. NKG2D signaling regulates IL-17A-producing gamma δT cells in mice to promote cancer progression Discovery Immunology 2022-05-10 [PMID: 36277678]
• J Spielmann, L Mattheis, JS Jung, H Rau beta e, M Gla beta , I Bähr, D Quandt, J Oswald, H Kielstein Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer Sci Rep, 2020-11-26;10(1):20606. 2020-11-26 [PMID: 33244094] (IHC, Mouse)
• C Godbersen-, TA Coupet, Z Grada, SC Zhang, CL Sentman Toxicity Induced by a Bispecific T Cell-Redirecting Protein Is Mediated by Both T Cells and Myeloid Cells in Immunocompetent Mice J. Immunol., 2020-04-15;204(11):2973-2983. 2020-04-15 [PMID: 32295875] (IHC, Mouse)
• Davies AJ, Kim HW, Gonzalez-Cano R et al. Natural Killer Cells Degenerate Intact Sensory Afferents following Nerve Injury Cell 2/7/2019 [PMID: 30712871]
• Kortlever Roderik M, Sodir Nicole M, Wilson Catherine H et al. Myc CoopeRates with Ras by Programming Inflammation and Immune Suppression. Cell 1/1/2017 [PMID: 29195074]
• T Kawakami, K Ito, Y Matsuda, M Noda, A Sakurada, Y Hoshikawa, Y Okada, K Ogasawara Cytotoxicity of natural killer cells activated through NKG2D contributes to the development of bronchiolitis obliterans in a murine heterotopic tracheal transplant model Am. J. Transplant, 2017-04-21;0(0):. 2017-04-21 [PMID: 28251796] (IHC-P, Mouse)
• X Qian, C Hu, S Han, Z Lin, W Xiao, Y Ding, Y Zhang, L Qian, X Jia, G Zhu, W Gong NK1.1(-) CD4(+) NKG2D(+) T cells suppress DSS-induced colitis in mice through production of TGF-? J. Cell. Mol. Med, 2017-02-22;0(0):. 2017-02-22 [PMID: 28224733] (IHC, Mouse)
• S Sheppard, J Guedes, A Mroz, AM Zavitsanou, H Kudo, SM Rothery, P Angelopoul, R Goldin, N Guerra The immunoreceptor NKG2D promotes tumour growth in a model of hepatocellular carcinoma Nat Commun, 2017-01-27;8(0):13930. 2017-01-27 [PMID: 28128200] (IHC, Mouse)
• Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery. J Leukoc Biol, 2015-01-12;97(3):583-98. 2015-01-12 [PMID: 25583577] (IHC, IHC-P, Human)
• Lin Z, Wang C, Xia H, Liu W, Xiao W, Qian L, Jia X, Ding Y, Ji M, Gong W CD4(+) NKG2D(+) T cells induce NKG2D down-regulation in natural killer cells in CD86-RAE-1epsilon transgenic mice. Immunology, 2014-03-01;141(3):401-15. 2014-03-01 [PMID: 24708417] (IHC, Western Blot, Mouse)
• Borchers MT, Wesselkamper SC, Curull V, Ramirez-Sarmiento A, Sanchez-Font A, Garcia-Aymerich J, Coronell C, Lloreta J, Agusti AG, Gea J, Howington JA, Reed MF, Starnes SL, Harris NL, Vitucci M, Eppert BL, Motz GT, Fogel K, McGraw DW, Tichelaar JW, Orozco-Levi M Sustained CTL activation by murine pulmonary epithelial cells promotes the development of COPD-like disease. J. Clin. Invest., 2009-02-09;119(3):636-49. 2009-02-09 [PMID: 19197141] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: Raet1a
• Uniprot:
  • O08604()