Rae-1 Antibody (199215) [Unconjugated] - Alpha/Beta/Gamma

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse Rae-1 alpha/beta/gamma Monoclonal Antibody (Catalog # MAB1758, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 199215
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rae-1 Antibody (199215) [Unconjugated] - Alpha/Beta/Gamma Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with Rae-1 beta and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ
• Specificity: Detects mouse Rae‑1 alpha / beta / gamma . When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody recognizes mouse Rae-1 alpha , beta and gamma . It does not recognize Rae-1δ or epsilon .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Raet1a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 Antibody (199215) [Unconjugated] - Alpha/Beta/Gamma

• dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1
• Rae1
• Rae-1

Background

The Rae-1 family is a group of closely related GPI-linked proteins that function as ligands for NKG2D. Binding of Rae-1 proteins to NKG2D results in the activation of cytolytic activity and/or cytokine production by effector T cells and NK cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rae-1 Antibody (MAB1758)(4)

Publications using MAB1758

• Y Tian, G Seumois, LM De-Oliveir, J Mateus, S Herrera-de, C Kim, D Hinz, NDS Goonawardh, AD de Silva, S Premawansa, G Premawansa, A Wijewickra, A Balmaseda, A Grifoni, P Vijayanand, E Harris, B Peters, A Sette, D Weiskopf Molecular Signatures of Dengue Virus-Specific IL-10/IFN-gamma Co-producing CD4�T Cells and Their Association with Dengue Disease Cell Rep, 2019-12-24;29(13):4482-4495.e4. 2019-12-24 [PMID: 31875555] (Flow Cytometry, Human)
• Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity. Leukemia, 2013-11-13;28(6):1316-25. 2013-11-13 [PMID: 24336127] (Flow Cytometry, Mouse)
• Ras activation induces expression of Raet1 family NK receptor ligands. J. Immunol., 2012-07-13;189(4):1826-34. 2012-07-13 [PMID: 22798674] (Flow Cytometry, Mouse)
• Hong X, Lei L, Kunert B, Naredla R, Applequist SE, Grandien A, Glas R Tripeptidyl-peptidase II controls DNA damage responses and in vivo gamma-irradiation resistance of tumors. Cancer Res., 2007-08-01;67(15):7165-74. 2007-08-01 [PMID: 17671184] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Raet1a