Rae-1 Antibody (199205) - Alpha/Beta/Delta/Epsilon

• Reactivity: Mu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 199205
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Rae-1 Antibody (199205) - Alpha/Beta/Delta/Epsilon Summary

• Immunogen: BaF3 mouse pro-B cell line transfected with Rae-1 beta (Accession # NP_033043) and Mouse myeloma cell line NS0-derived recombinant mouse Rae-1δ Leu29-Ser227 (Accession # Q9JI58)
• Specificity: Detects mouse Rae‑1 alpha / beta /δ/ epsilon . When tested in flow cytometry against a panel of transfectants expressing different Rae-1 proteins, this antibody strongly recognizes Rae-1 beta and Rae-1δ. It shows weaker staining of Rae-1 alpha and Rae-1 epsilon transfectants. It does not recognize Rae-1 gamma .
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Raet1a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Rae-1 Antibody (199205) - Alpha/Beta/Delta/Epsilon

• dJ481F12.3;dJ800J21.1;Gle2;MIG14;Mnrp41;mRNA export factor RAE1;MRNP41;RAE1
• Rae1
• Rae-1

Background

Rae-1 alpha , beta , gamma , δ and epsilon (retinoic acid early transcript 1) comprise a family of closely related (88‑95% amino acid identity) GPI-linked cell surface proteins that function as ligands for mouse NKG2D, an activating receptor expressed on NK and T cells. Rae-1 transcripts are expressed in mouse embryos and several tumor cell lines but are absent from most normal adult tissues. Rae-1 protein expression on tumor cell lines has been implicated in in vivo tumor rejection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Rae-1 Antibody (MAB17581)(4)

Publications using MAB17581

• Li Hua, Mingli Fang, Boqi Dong, Sheng Guo, Cuiyun Cui, Jiwei Liu et al. Attribution of NKG2DL to the inhibition of early stage allogeneic tumors in mice Oncotarget 2016-12-13 [PMID: 27448968]
• Ras activation induces expression of Raet1 family NK receptor ligands. J. Immunol., 2012-07-13;189(4):1826-34. 2012-07-13 [PMID: 22798674] (Flow Cytometry, Mouse)
• Yoshida S, Mohamed RH, Kajikawa M, Koizumi J, Tanaka M, Fugo K, Otsuka N, Maenaka K, Yagita H, Chiba H, Kasahara M Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair. J. Immunol., 2012-03-07;188(8):3972-9. 2012-03-07 [PMID: 22403443] (IHC-Fr, Mouse)
• Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. PLoS Pathog., 2012-01-12;8(1):e1002481. 2012-01-12 [PMID: 22253596] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: Raet1a