RACK1/GNB2L1 Antibody (JG40-26)

Western Blot: RACK1/GNB2L1 Antibody (JG40-26) [NBP2-76864] - Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2,000 dilution.Positive control:Lane 1: SH-SY-5Y Lane 2: Rat small intestine

Immunohistochemistry: RACK1/GNB2L1 Antibody (JG40-26) [NBP2-76864] - Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-RACK1 antibody. Counter stained with hematoxylin.

Flow Cytometry: RACK1/GNB2L1 Antibody (JG40-26) [NBP2-76864] - Flow cytometric analysis of SH-SY-5Y cells with RACK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunohistochemistry: RACK1/GNB2L1 Antibody (JG40-26) [NBP2-76864] - Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: RACK1/GNB2L1 Antibody (JG40-26) [NBP2-76864] - Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-RACK1 antibody. Counter stained with hematoxylin.

Immunohistochemistry: RACK1/GNB2L1 Antibody (JG40-26) [NBP2-76864] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RACK1 antibody. Counter stained with hematoxylin.

Product Discontinued

RACK1/GNB2L1 Antibody (JG40-26) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human RACK1/GNB2L1 aa 190-317 / 317. (SwissProt: P63244 Human; SwissProt: P63245 Rat)
• Localization: Cytoplasm. Cell membrane. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RACK1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for RACK1/GNB2L1 Antibody (JG40-26)

• GNB2L1
• guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• guanine nucleotide binding
• RACK1
• Receptor for Activated C Kinase 1
• Receptor for activated C kinase

Background

RACK1 (receptor for activated C kinase 1) was identified through its binding to various PKC isoforms. Its main function is to recruit PKC and various other proteins to specific location to form multiprotein complexes, mediating various signal pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RACK1